Neutral Red-carbon nanodots for selective fluorescent DNA sensing

Carbon nanodots modified with Neutral Red covalently inserted in the nanostructure (NR-CDs) have been prepared by a simple synthesis method based on microwave irradiation under controlled temperature and pressure. The synthetized NR-CDs have been characterized by different techniques, demonstrating...

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Detalles Bibliográficos
Autores principales: Martínez-Periñán, Emiliano, Martínez-Sobrino, Álvaro, Bravo, Iria, García-Mendiola, Tania, Mateo-Martí, Eva, Pariente, Félix, Lorenzo, Encarnación
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242914/
https://www.ncbi.nlm.nih.gov/pubmed/35288763
http://dx.doi.org/10.1007/s00216-022-03980-1
Descripción
Sumario:Carbon nanodots modified with Neutral Red covalently inserted in the nanostructure (NR-CDs) have been prepared by a simple synthesis method based on microwave irradiation under controlled temperature and pressure. The synthetized NR-CDs have been characterized by different techniques, demonstrating the covalent bonding of Neutral Red molecules to the carbon dots nanostructure. Fluorescence activity of the prepare NR-CDs has been explored showing different interaction pathways with singled and doubled stranded DNA. These studies have been successfully applied to develop a new fluorescence DNA hybridization assay to the detection of a specific DNA sequence of Escherichia coli bacteria. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-03980-1.

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