Neutral Red-carbon nanodots for selective fluorescent DNA sensing

Carbon nanodots modified with Neutral Red covalently inserted in the nanostructure (NR-CDs) have been prepared by a simple synthesis method based on microwave irradiation under controlled temperature and pressure. The synthetized NR-CDs have been characterized by different techniques, demonstrating...

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Autores principales: Martínez-Periñán, Emiliano, Martínez-Sobrino, Álvaro, Bravo, Iria, García-Mendiola, Tania, Mateo-Martí, Eva, Pariente, Félix, Lorenzo, Encarnación
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242914/
https://www.ncbi.nlm.nih.gov/pubmed/35288763
http://dx.doi.org/10.1007/s00216-022-03980-1
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author Martínez-Periñán, Emiliano
Martínez-Sobrino, Álvaro
Bravo, Iria
García-Mendiola, Tania
Mateo-Martí, Eva
Pariente, Félix
Lorenzo, Encarnación
author_facet Martínez-Periñán, Emiliano
Martínez-Sobrino, Álvaro
Bravo, Iria
García-Mendiola, Tania
Mateo-Martí, Eva
Pariente, Félix
Lorenzo, Encarnación
author_sort Martínez-Periñán, Emiliano
collection PubMed
description Carbon nanodots modified with Neutral Red covalently inserted in the nanostructure (NR-CDs) have been prepared by a simple synthesis method based on microwave irradiation under controlled temperature and pressure. The synthetized NR-CDs have been characterized by different techniques, demonstrating the covalent bonding of Neutral Red molecules to the carbon dots nanostructure. Fluorescence activity of the prepare NR-CDs has been explored showing different interaction pathways with singled and doubled stranded DNA. These studies have been successfully applied to develop a new fluorescence DNA hybridization assay to the detection of a specific DNA sequence of Escherichia coli bacteria. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-03980-1.
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spelling pubmed-92429142022-07-01 Neutral Red-carbon nanodots for selective fluorescent DNA sensing Martínez-Periñán, Emiliano Martínez-Sobrino, Álvaro Bravo, Iria García-Mendiola, Tania Mateo-Martí, Eva Pariente, Félix Lorenzo, Encarnación Anal Bioanal Chem Research Paper Carbon nanodots modified with Neutral Red covalently inserted in the nanostructure (NR-CDs) have been prepared by a simple synthesis method based on microwave irradiation under controlled temperature and pressure. The synthetized NR-CDs have been characterized by different techniques, demonstrating the covalent bonding of Neutral Red molecules to the carbon dots nanostructure. Fluorescence activity of the prepare NR-CDs has been explored showing different interaction pathways with singled and doubled stranded DNA. These studies have been successfully applied to develop a new fluorescence DNA hybridization assay to the detection of a specific DNA sequence of Escherichia coli bacteria. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-03980-1. Springer Berlin Heidelberg 2022-03-14 2022 /pmc/articles/PMC9242914/ /pubmed/35288763 http://dx.doi.org/10.1007/s00216-022-03980-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Paper
Martínez-Periñán, Emiliano
Martínez-Sobrino, Álvaro
Bravo, Iria
García-Mendiola, Tania
Mateo-Martí, Eva
Pariente, Félix
Lorenzo, Encarnación
Neutral Red-carbon nanodots for selective fluorescent DNA sensing
title Neutral Red-carbon nanodots for selective fluorescent DNA sensing
title_full Neutral Red-carbon nanodots for selective fluorescent DNA sensing
title_fullStr Neutral Red-carbon nanodots for selective fluorescent DNA sensing
title_full_unstemmed Neutral Red-carbon nanodots for selective fluorescent DNA sensing
title_short Neutral Red-carbon nanodots for selective fluorescent DNA sensing
title_sort neutral red-carbon nanodots for selective fluorescent dna sensing
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242914/
https://www.ncbi.nlm.nih.gov/pubmed/35288763
http://dx.doi.org/10.1007/s00216-022-03980-1
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