Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy

Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8(+) T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIV(SF162P3)-infected rhesus ma...

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Detalles Bibliográficos
Autores principales: Sun, Yuting, Zhang, Jingjing, Tong, Ling, Lu, Jiahan, Chen, Ting, Wei, Qiang, He, Yuxian, Cong, Zhe, Xue, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243296/
https://www.ncbi.nlm.nih.gov/pubmed/35776642
http://dx.doi.org/10.1016/j.xpro.2022.101479
Descripción
Sumario:Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8(+) T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIV(SF162P3)-infected rhesus macaques with an HIV fusion-inhibitory lipopeptide (LP-98) monotherapy. We also present the steps for adoptive transfer of an anti-CD8 antibody into a stable virologic control (SVC) group of LP-98-treated monkeys, confirming a direct role of CD8(+) T cells in SVC macaques. For complete details on the use and execution of this protocol, please refer to Xue et al. (2022).

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