Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy

Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8(+) T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIV(SF162P3)-infected rhesus ma...

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Autores principales: Sun, Yuting, Zhang, Jingjing, Tong, Ling, Lu, Jiahan, Chen, Ting, Wei, Qiang, He, Yuxian, Cong, Zhe, Xue, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243296/
https://www.ncbi.nlm.nih.gov/pubmed/35776642
http://dx.doi.org/10.1016/j.xpro.2022.101479
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author Sun, Yuting
Zhang, Jingjing
Tong, Ling
Lu, Jiahan
Chen, Ting
Wei, Qiang
He, Yuxian
Cong, Zhe
Xue, Jing
author_facet Sun, Yuting
Zhang, Jingjing
Tong, Ling
Lu, Jiahan
Chen, Ting
Wei, Qiang
He, Yuxian
Cong, Zhe
Xue, Jing
author_sort Sun, Yuting
collection PubMed
description Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8(+) T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIV(SF162P3)-infected rhesus macaques with an HIV fusion-inhibitory lipopeptide (LP-98) monotherapy. We also present the steps for adoptive transfer of an anti-CD8 antibody into a stable virologic control (SVC) group of LP-98-treated monkeys, confirming a direct role of CD8(+) T cells in SVC macaques. For complete details on the use and execution of this protocol, please refer to Xue et al. (2022).
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spelling pubmed-92432962022-07-01 Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy Sun, Yuting Zhang, Jingjing Tong, Ling Lu, Jiahan Chen, Ting Wei, Qiang He, Yuxian Cong, Zhe Xue, Jing STAR Protoc Protocol Strong cellular immunity contributes to the control of HIV infection. Here, we describe a step-by-step protocol to assess the simian immunodeficiency virus (SIV)-specific CD8(+) T cell responses by quantifying the degranulation, cytokine and chemokine production from SHIV(SF162P3)-infected rhesus macaques with an HIV fusion-inhibitory lipopeptide (LP-98) monotherapy. We also present the steps for adoptive transfer of an anti-CD8 antibody into a stable virologic control (SVC) group of LP-98-treated monkeys, confirming a direct role of CD8(+) T cells in SVC macaques. For complete details on the use and execution of this protocol, please refer to Xue et al. (2022). Elsevier 2022-06-22 /pmc/articles/PMC9243296/ /pubmed/35776642 http://dx.doi.org/10.1016/j.xpro.2022.101479 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sun, Yuting
Zhang, Jingjing
Tong, Ling
Lu, Jiahan
Chen, Ting
Wei, Qiang
He, Yuxian
Cong, Zhe
Xue, Jing
Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy
title Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy
title_full Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy
title_fullStr Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy
title_full_unstemmed Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy
title_short Protocol for evaluating CD8(+) T cell-mediated immunity in latently SHIV-infected rhesus macaques with HIV fusion-inhibitory lipopeptide monotherapy
title_sort protocol for evaluating cd8(+) t cell-mediated immunity in latently shiv-infected rhesus macaques with hiv fusion-inhibitory lipopeptide monotherapy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243296/
https://www.ncbi.nlm.nih.gov/pubmed/35776642
http://dx.doi.org/10.1016/j.xpro.2022.101479
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