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Human obstructive (postvasectomy) and nonobstructive azoospermia – Insights from scRNA-Seq and transcriptome analysis

A substantial number of male infertility is caused by azoospermia. However, the underlying etiology and the molecular basis remain largely unknown. Through single-cell (sc)RNA sequencing, we had analyzed testis biopsy samples from two patients with obstructive azoospermia (OA) and nonobstructive azo...

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Detalles Bibliográficos
Autores principales: Chen, Shitao, An, Geng, Wang, Hanshu, Wu, Xiaolong, Ping, Ping, Hu, Longfei, Chen, Yunmei, Fan, Jue, Cheng, C. Yan, Sun, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chongqing Medical University 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243341/
https://www.ncbi.nlm.nih.gov/pubmed/35782978
http://dx.doi.org/10.1016/j.gendis.2020.09.004
Descripción
Sumario:A substantial number of male infertility is caused by azoospermia. However, the underlying etiology and the molecular basis remain largely unknown. Through single-cell (sc)RNA sequencing, we had analyzed testis biopsy samples from two patients with obstructive azoospermia (OA) and nonobstructive azoospermia (NOA). We found only somatic cells in the NOA samples and explored the transcriptional changes in Sertoli cells in response to a loss of interactions with germ cells. Moreover, we observed a germ cell population discrepancy between an OA (postvasectomy) patient and a healthy individual. We confirmed this observation in a secondary study with two datasets at GSM3526588 and GSE124263 for detailed analysis wherein the regulatory mechanisms at the transcriptional level were identified. These findings thus provide valuable information on human spermatogenesis, and we also identified insightful information for further research on reproduction-related diseases.