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Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer

Branched-chain amino acids (BCAAs) are the three essential amino acids including leucine, isoleucine, and valine. BCAA metabolism has been linked with the development of a variety of tumors. However, the impact of dietary BCAA intake on breast tumor progression and metastasis remains to be fully exp...

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Autores principales: Chi, Rui, Yao, Chengcheng, Chen, Si, Liu, Yunxia, He, Yanqi, Zhang, Jin, Ellies, Lesley G., Wu, Xuefeng, Zhao, Qian, Zhou, Cixiang, Wang, Ying, Sun, Haipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243538/
https://www.ncbi.nlm.nih.gov/pubmed/35785192
http://dx.doi.org/10.3389/fonc.2022.887257
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author Chi, Rui
Yao, Chengcheng
Chen, Si
Liu, Yunxia
He, Yanqi
Zhang, Jin
Ellies, Lesley G.
Wu, Xuefeng
Zhao, Qian
Zhou, Cixiang
Wang, Ying
Sun, Haipeng
author_facet Chi, Rui
Yao, Chengcheng
Chen, Si
Liu, Yunxia
He, Yanqi
Zhang, Jin
Ellies, Lesley G.
Wu, Xuefeng
Zhao, Qian
Zhou, Cixiang
Wang, Ying
Sun, Haipeng
author_sort Chi, Rui
collection PubMed
description Branched-chain amino acids (BCAAs) are the three essential amino acids including leucine, isoleucine, and valine. BCAA metabolism has been linked with the development of a variety of tumors. However, the impact of dietary BCAA intake on breast tumor progression and metastasis remains to be fully explored. Here, we unexpectedly find that the elevated BCAA, either in the genetic model or via increasing dietary intake in mice, suppresses the tumor growth and lung metastasis of breast cancer. The survival analysis shows that BCAA catabolic gene expression is strongly associated with long-term oncological outcomes in patients with breast cancer. In Pp2cm knockout mice in which BCAAs accumulate due to the genetic defect of BCAA catabolism, the breast tumor growth is suppressed. Interestingly, while the cell proliferation and tumor vasculature remain unaffected, more cell death occurs in the tumor in Pp2cm knockout mice, accompanied with increased natural killer (NK) cells. Importantly, increasing BCAA dietary intake suppresses breast tumor growth in mice. On the other hand, there are fewer lung metastases from primary breast tumor in Pp2cm knockout mice and the high BCAA diet-fed mice, suggesting high BCAA also suppresses the lung metastasis of breast cancer. Furthermore, low BCAA diet promotes lung colonization of breast cancer cells in tail vein model. The migration and invasion abilities of breast cancer cells are impaired by high concentration of BCAA in culture medium. The suppressed tumor metastasis and cell migration/invasion abilities by elevated BCAA are accompanied with reduced N-cadherin expression. Together, these data show high BCAA suppresses both tumor growth and metastasis of breast cancer, demonstrating the potential benefits of increasing BCAA dietary intake in the treatment of breast cancer.
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spelling pubmed-92435382022-07-01 Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer Chi, Rui Yao, Chengcheng Chen, Si Liu, Yunxia He, Yanqi Zhang, Jin Ellies, Lesley G. Wu, Xuefeng Zhao, Qian Zhou, Cixiang Wang, Ying Sun, Haipeng Front Oncol Oncology Branched-chain amino acids (BCAAs) are the three essential amino acids including leucine, isoleucine, and valine. BCAA metabolism has been linked with the development of a variety of tumors. However, the impact of dietary BCAA intake on breast tumor progression and metastasis remains to be fully explored. Here, we unexpectedly find that the elevated BCAA, either in the genetic model or via increasing dietary intake in mice, suppresses the tumor growth and lung metastasis of breast cancer. The survival analysis shows that BCAA catabolic gene expression is strongly associated with long-term oncological outcomes in patients with breast cancer. In Pp2cm knockout mice in which BCAAs accumulate due to the genetic defect of BCAA catabolism, the breast tumor growth is suppressed. Interestingly, while the cell proliferation and tumor vasculature remain unaffected, more cell death occurs in the tumor in Pp2cm knockout mice, accompanied with increased natural killer (NK) cells. Importantly, increasing BCAA dietary intake suppresses breast tumor growth in mice. On the other hand, there are fewer lung metastases from primary breast tumor in Pp2cm knockout mice and the high BCAA diet-fed mice, suggesting high BCAA also suppresses the lung metastasis of breast cancer. Furthermore, low BCAA diet promotes lung colonization of breast cancer cells in tail vein model. The migration and invasion abilities of breast cancer cells are impaired by high concentration of BCAA in culture medium. The suppressed tumor metastasis and cell migration/invasion abilities by elevated BCAA are accompanied with reduced N-cadherin expression. Together, these data show high BCAA suppresses both tumor growth and metastasis of breast cancer, demonstrating the potential benefits of increasing BCAA dietary intake in the treatment of breast cancer. Frontiers Media S.A. 2022-06-16 /pmc/articles/PMC9243538/ /pubmed/35785192 http://dx.doi.org/10.3389/fonc.2022.887257 Text en Copyright © 2022 Chi, Yao, Chen, Liu, He, Zhang, Ellies, Wu, Zhao, Zhou, Wang and Sun https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Chi, Rui
Yao, Chengcheng
Chen, Si
Liu, Yunxia
He, Yanqi
Zhang, Jin
Ellies, Lesley G.
Wu, Xuefeng
Zhao, Qian
Zhou, Cixiang
Wang, Ying
Sun, Haipeng
Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer
title Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer
title_full Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer
title_fullStr Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer
title_full_unstemmed Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer
title_short Elevated BCAA Suppresses the Development and Metastasis of Breast Cancer
title_sort elevated bcaa suppresses the development and metastasis of breast cancer
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243538/
https://www.ncbi.nlm.nih.gov/pubmed/35785192
http://dx.doi.org/10.3389/fonc.2022.887257
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