Efficient assembly of a large fragment of monkeypox virus genome as a qPCR template using dual-selection based transformation-associated recombination

Transformation-associated recombination (TAR) has been widely used to assemble large DNA constructs. One of the significant obstacles hindering assembly efficiency is the presence of error-prone DNA repair pathways in yeast, which results in vector backbone recircularization or illegitimate recombin...

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Detalles Bibliográficos
Autores principales: Yang, Lei, Tian, Lingqian, Li, Leshan, Liu, Qiuhong, Guo, Xiang, Zhou, Yuan, Pei, Rongjuan, Chen, Xinwen, Wang, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243609/
https://www.ncbi.nlm.nih.gov/pubmed/35393265
http://dx.doi.org/10.1016/j.virs.2022.02.009

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