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Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli
Uropathogenic Escherichia coli (UPEC) is a nosocomial pathogen associated with urinary tract infections and expresses several virulence factors that cause recurring infections and cystitis of the bladder, which can lead to pyelonephritis. UPEC uses different types of extracellular appendages like fi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244173/ https://www.ncbi.nlm.nih.gov/pubmed/35783430 http://dx.doi.org/10.3389/fmicb.2022.872943 |
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author | Boya, Bharath Reddy Lee, Jin-Hyung Lee, Jintae |
author_facet | Boya, Bharath Reddy Lee, Jin-Hyung Lee, Jintae |
author_sort | Boya, Bharath Reddy |
collection | PubMed |
description | Uropathogenic Escherichia coli (UPEC) is a nosocomial pathogen associated with urinary tract infections and expresses several virulence factors that cause recurring infections and cystitis of the bladder, which can lead to pyelonephritis. UPEC uses different types of extracellular appendages like fimbriae and pili that aid colonization and adherence to bladder epithelium and can form persistent biofilm-like bacterial communities that aid its survival after the deployment of host immune responses. We investigated the antibiofilm, antimicrobial, and antivirulence properties of three indole derivatives namely, 4-chloroindole, 5-chloroindole, and 5-chloro 2-methyl indole. All the three chloroindoles had MICs of 75 μg/ml and inhibited biofilm formation by an average of 67% at 20 μg/ml. In addition, they inhibited swarming and swimming motilities, which are essential for dissemination from bacterial communities and colonization, reduced cell surface hydrophobicity, and inhibited indole production and curli formation. Gene expression analysis showed all three chloroindoles significantly downregulated the expressions of virulence genes associated with adhesion, stress regulation, and toxin production. A 3D-QSAR analysis revealed substitutions at the fourth and fifth positions of the indole moiety favored antimicrobial activity. Furthermore, these chloroindoles potently inhibited biofilm formation in other nosocomial pathogens and polymicrobial consortia. |
format | Online Article Text |
id | pubmed-9244173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92441732022-07-01 Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli Boya, Bharath Reddy Lee, Jin-Hyung Lee, Jintae Front Microbiol Microbiology Uropathogenic Escherichia coli (UPEC) is a nosocomial pathogen associated with urinary tract infections and expresses several virulence factors that cause recurring infections and cystitis of the bladder, which can lead to pyelonephritis. UPEC uses different types of extracellular appendages like fimbriae and pili that aid colonization and adherence to bladder epithelium and can form persistent biofilm-like bacterial communities that aid its survival after the deployment of host immune responses. We investigated the antibiofilm, antimicrobial, and antivirulence properties of three indole derivatives namely, 4-chloroindole, 5-chloroindole, and 5-chloro 2-methyl indole. All the three chloroindoles had MICs of 75 μg/ml and inhibited biofilm formation by an average of 67% at 20 μg/ml. In addition, they inhibited swarming and swimming motilities, which are essential for dissemination from bacterial communities and colonization, reduced cell surface hydrophobicity, and inhibited indole production and curli formation. Gene expression analysis showed all three chloroindoles significantly downregulated the expressions of virulence genes associated with adhesion, stress regulation, and toxin production. A 3D-QSAR analysis revealed substitutions at the fourth and fifth positions of the indole moiety favored antimicrobial activity. Furthermore, these chloroindoles potently inhibited biofilm formation in other nosocomial pathogens and polymicrobial consortia. Frontiers Media S.A. 2022-06-16 /pmc/articles/PMC9244173/ /pubmed/35783430 http://dx.doi.org/10.3389/fmicb.2022.872943 Text en Copyright © 2022 Boya, Lee and Lee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Boya, Bharath Reddy Lee, Jin-Hyung Lee, Jintae Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli |
title | Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli |
title_full | Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli |
title_fullStr | Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli |
title_full_unstemmed | Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli |
title_short | Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli |
title_sort | antibiofilm and antimicrobial activities of chloroindoles against uropathogenic escherichia coli |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244173/ https://www.ncbi.nlm.nih.gov/pubmed/35783430 http://dx.doi.org/10.3389/fmicb.2022.872943 |
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