Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry

The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high‐throughput screenings. Here, we present Listeria sp...

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Detalles Bibliográficos
Autores principales: Reich, Sebastian J., Stohr, Jonas, Goldbeck, Oliver, Fendrich, Bastian, Crauwels, Peter, Riedel, Christian U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Commentary
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245168/
https://www.ncbi.nlm.nih.gov/pubmed/36031957
http://dx.doi.org/10.1002/mbo3.1304
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author Reich, Sebastian J.
Stohr, Jonas
Goldbeck, Oliver
Fendrich, Bastian
Crauwels, Peter
Riedel, Christian U.
author_facet Reich, Sebastian J.
Stohr, Jonas
Goldbeck, Oliver
Fendrich, Bastian
Crauwels, Peter
Riedel, Christian U.
author_sort Reich, Sebastian J.
collection PubMed
description The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high‐throughput screenings. Here, we present Listeria spp. pH‐sensitive biosensors with improved fluorescence for single‐cell analysis of antimicrobials by flow cytometry.
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spelling pubmed-92451682022-07-01 Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry Reich, Sebastian J. Stohr, Jonas Goldbeck, Oliver Fendrich, Bastian Crauwels, Peter Riedel, Christian U. Microbiologyopen Commentary The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high‐throughput screenings. Here, we present Listeria spp. pH‐sensitive biosensors with improved fluorescence for single‐cell analysis of antimicrobials by flow cytometry. John Wiley and Sons Inc. 2022-06-30 /pmc/articles/PMC9245168/ /pubmed/36031957 http://dx.doi.org/10.1002/mbo3.1304 Text en © 2022 The Authors. Microbiology Open published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Commentary
Reich, Sebastian J.
Stohr, Jonas
Goldbeck, Oliver
Fendrich, Bastian
Crauwels, Peter
Riedel, Christian U.
Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
title Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
title_full Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
title_fullStr Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
title_full_unstemmed Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
title_short Improved fluorescent Listeria spp. biosensors for analysis of antimicrobials by flow cytometry
title_sort improved fluorescent listeria spp. biosensors for analysis of antimicrobials by flow cytometry
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245168/
https://www.ncbi.nlm.nih.gov/pubmed/36031957
http://dx.doi.org/10.1002/mbo3.1304
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