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Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells

[Image: see text] Artificial cells are minimal structures constructed from biomolecular building blocks designed to mimic cellular processes, behaviors, and architectures. One near-ubiquitous feature of cellular life is the spatial organization of internal content. We know from biology that organiza...

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Autores principales: Zubaite, Greta, Hindley, James W., Ces, Oscar, Elani, Yuval
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245354/
https://www.ncbi.nlm.nih.gov/pubmed/35695383
http://dx.doi.org/10.1021/acsnano.2c02195
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author Zubaite, Greta
Hindley, James W.
Ces, Oscar
Elani, Yuval
author_facet Zubaite, Greta
Hindley, James W.
Ces, Oscar
Elani, Yuval
author_sort Zubaite, Greta
collection PubMed
description [Image: see text] Artificial cells are minimal structures constructed from biomolecular building blocks designed to mimic cellular processes, behaviors, and architectures. One near-ubiquitous feature of cellular life is the spatial organization of internal content. We know from biology that organization of content (including in membrane-bound organelles) is linked to cellular functions and that this feature is dynamic: the presence, location, and degree of compartmentalization changes over time. Vesicle-based artificial cells, however, are not currently able to mimic this fundamental cellular property. Here, we describe an artificial cell design strategy that addresses this technological bottleneck. We create a series of artificial cell architectures which possess multicompartment assemblies localized either on the inner or on the outer surface of the artificial cell membrane. Exploiting liquid–liquid phase separation, we can also engineer spatially segregated regions of condensed subcompartments attached to the cell surface, aligning with coexisting membrane domains. These structures can sense changes in environmental conditions and respond by reversibly transitioning from condensed multicompartment layers on the membrane surface to a dispersed state in the cell lumen, mimicking the dynamic compartmentalization found in biological cells. Likewise, we engineer exosome-like subcompartments that can be released to the environment. We can achieve this by using two types of triggers: chemical (addition of salts) and mechanical (by pulling membrane tethers using optical traps). These approaches allow us to control the compartmentalization state of artificial cells on population and single-cell levels.
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spelling pubmed-92453542022-07-01 Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells Zubaite, Greta Hindley, James W. Ces, Oscar Elani, Yuval ACS Nano [Image: see text] Artificial cells are minimal structures constructed from biomolecular building blocks designed to mimic cellular processes, behaviors, and architectures. One near-ubiquitous feature of cellular life is the spatial organization of internal content. We know from biology that organization of content (including in membrane-bound organelles) is linked to cellular functions and that this feature is dynamic: the presence, location, and degree of compartmentalization changes over time. Vesicle-based artificial cells, however, are not currently able to mimic this fundamental cellular property. Here, we describe an artificial cell design strategy that addresses this technological bottleneck. We create a series of artificial cell architectures which possess multicompartment assemblies localized either on the inner or on the outer surface of the artificial cell membrane. Exploiting liquid–liquid phase separation, we can also engineer spatially segregated regions of condensed subcompartments attached to the cell surface, aligning with coexisting membrane domains. These structures can sense changes in environmental conditions and respond by reversibly transitioning from condensed multicompartment layers on the membrane surface to a dispersed state in the cell lumen, mimicking the dynamic compartmentalization found in biological cells. Likewise, we engineer exosome-like subcompartments that can be released to the environment. We can achieve this by using two types of triggers: chemical (addition of salts) and mechanical (by pulling membrane tethers using optical traps). These approaches allow us to control the compartmentalization state of artificial cells on population and single-cell levels. American Chemical Society 2022-06-13 2022-06-28 /pmc/articles/PMC9245354/ /pubmed/35695383 http://dx.doi.org/10.1021/acsnano.2c02195 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Zubaite, Greta
Hindley, James W.
Ces, Oscar
Elani, Yuval
Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells
title Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells
title_full Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells
title_fullStr Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells
title_full_unstemmed Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells
title_short Dynamic Reconfiguration of Subcompartment Architectures in Artificial Cells
title_sort dynamic reconfiguration of subcompartment architectures in artificial cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245354/
https://www.ncbi.nlm.nih.gov/pubmed/35695383
http://dx.doi.org/10.1021/acsnano.2c02195
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