Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of...

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Autores principales: Tavakoli, Kamand, Pour-Aboughadareh, Alireza, Kianersi, Farzad, Poczai, Peter, Etminan, Alireza, Shooshtari, Lia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245484/
https://www.ncbi.nlm.nih.gov/pubmed/35822768
http://dx.doi.org/10.3390/biotech10030014
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author Tavakoli, Kamand
Pour-Aboughadareh, Alireza
Kianersi, Farzad
Poczai, Peter
Etminan, Alireza
Shooshtari, Lia
author_facet Tavakoli, Kamand
Pour-Aboughadareh, Alireza
Kianersi, Farzad
Poczai, Peter
Etminan, Alireza
Shooshtari, Lia
author_sort Tavakoli, Kamand
collection PubMed
description Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.
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spelling pubmed-92454842022-07-06 Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences Tavakoli, Kamand Pour-Aboughadareh, Alireza Kianersi, Farzad Poczai, Peter Etminan, Alireza Shooshtari, Lia BioTech (Basel) Review Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding. MDPI 2021-07-06 /pmc/articles/PMC9245484/ /pubmed/35822768 http://dx.doi.org/10.3390/biotech10030014 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Tavakoli, Kamand
Pour-Aboughadareh, Alireza
Kianersi, Farzad
Poczai, Peter
Etminan, Alireza
Shooshtari, Lia
Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_full Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_fullStr Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_full_unstemmed Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_short Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences
title_sort applications of crispr-cas9 as an advanced genome editing system in life sciences
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245484/
https://www.ncbi.nlm.nih.gov/pubmed/35822768
http://dx.doi.org/10.3390/biotech10030014
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