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Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms

While biofilms formed by bacteria have received great attention due to their importance in pathogenesis, much less research has been focused on the biofilms formed by archaea. It has been known that extracellular filaments in archaea, such as type IV pili, hami, and cannulae, play a part in the form...

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Autores principales: Wang, Fengbin, Cvirkaite-Krupovic, Virginija, Krupovic, Mart, Egelman, Edward H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245690/
https://www.ncbi.nlm.nih.gov/pubmed/35727984
http://dx.doi.org/10.1073/pnas.2207037119
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author Wang, Fengbin
Cvirkaite-Krupovic, Virginija
Krupovic, Mart
Egelman, Edward H.
author_facet Wang, Fengbin
Cvirkaite-Krupovic, Virginija
Krupovic, Mart
Egelman, Edward H.
author_sort Wang, Fengbin
collection PubMed
description While biofilms formed by bacteria have received great attention due to their importance in pathogenesis, much less research has been focused on the biofilms formed by archaea. It has been known that extracellular filaments in archaea, such as type IV pili, hami, and cannulae, play a part in the formation of archaeal biofilms. We have used cryo-electron microscopy to determine the atomic structure of a previously uncharacterized class of archaeal surface filaments from hyperthermophilic Pyrobaculum calidifontis. These filaments, which we call archaeal bundling pili (ABP), assemble into highly ordered bipolar bundles. The bipolar nature of these bundles most likely arises from the association of filaments from at least two different cells. The component protein, AbpA, shows homology, both at the sequence and structural level, to the bacterial protein TasA, a major component of the extracellular matrix in bacterial biofilms, contributing to biofilm stability. We show that AbpA forms very stable filaments in a manner similar to the donor-strand exchange of bacterial TasA fibers and chaperone-usher pathway pili where a β-strand from one subunit is incorporated into a β-sheet of the next subunit. Our results reveal likely mechanistic similarities and evolutionary connection between bacterial and archaeal biofilms, and suggest that there could be many other archaeal surface filaments that are as yet uncharacterized.
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spelling pubmed-92456902022-12-21 Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms Wang, Fengbin Cvirkaite-Krupovic, Virginija Krupovic, Mart Egelman, Edward H. Proc Natl Acad Sci U S A Biological Sciences While biofilms formed by bacteria have received great attention due to their importance in pathogenesis, much less research has been focused on the biofilms formed by archaea. It has been known that extracellular filaments in archaea, such as type IV pili, hami, and cannulae, play a part in the formation of archaeal biofilms. We have used cryo-electron microscopy to determine the atomic structure of a previously uncharacterized class of archaeal surface filaments from hyperthermophilic Pyrobaculum calidifontis. These filaments, which we call archaeal bundling pili (ABP), assemble into highly ordered bipolar bundles. The bipolar nature of these bundles most likely arises from the association of filaments from at least two different cells. The component protein, AbpA, shows homology, both at the sequence and structural level, to the bacterial protein TasA, a major component of the extracellular matrix in bacterial biofilms, contributing to biofilm stability. We show that AbpA forms very stable filaments in a manner similar to the donor-strand exchange of bacterial TasA fibers and chaperone-usher pathway pili where a β-strand from one subunit is incorporated into a β-sheet of the next subunit. Our results reveal likely mechanistic similarities and evolutionary connection between bacterial and archaeal biofilms, and suggest that there could be many other archaeal surface filaments that are as yet uncharacterized. National Academy of Sciences 2022-06-21 2022-06-28 /pmc/articles/PMC9245690/ /pubmed/35727984 http://dx.doi.org/10.1073/pnas.2207037119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Wang, Fengbin
Cvirkaite-Krupovic, Virginija
Krupovic, Mart
Egelman, Edward H.
Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms
title Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms
title_full Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms
title_fullStr Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms
title_full_unstemmed Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms
title_short Archaeal bundling pili of Pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms
title_sort archaeal bundling pili of pyrobaculum calidifontis reveal similarities between archaeal and bacterial biofilms
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245690/
https://www.ncbi.nlm.nih.gov/pubmed/35727984
http://dx.doi.org/10.1073/pnas.2207037119
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