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Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population

The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard se...

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Autores principales: Tao, Ruiyang, Chen, Chong, Sheng, Xiang, Xia, Ruocheng, Zhang, Xiaochun, Zhang, Jingyi, Yang, Zihao, Zhang, Suhua, Li, Chengtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246015/
https://www.ncbi.nlm.nih.gov/pubmed/35784410
http://dx.doi.org/10.1080/20961790.2019.1665160
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author Tao, Ruiyang
Chen, Chong
Sheng, Xiang
Xia, Ruocheng
Zhang, Xiaochun
Zhang, Jingyi
Yang, Zihao
Zhang, Suhua
Li, Chengtao
author_facet Tao, Ruiyang
Chen, Chong
Sheng, Xiang
Xia, Ruocheng
Zhang, Xiaochun
Zhang, Jingyi
Yang, Zihao
Zhang, Suhua
Li, Chengtao
author_sort Tao, Ruiyang
collection PubMed
description The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.
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spelling pubmed-92460152022-07-01 Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population Tao, Ruiyang Chen, Chong Sheng, Xiang Xia, Ruocheng Zhang, Xiaochun Zhang, Jingyi Yang, Zihao Zhang, Suhua Li, Chengtao Forensic Sci Res Original Articles The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population. Taylor & Francis 2019-12-06 /pmc/articles/PMC9246015/ /pubmed/35784410 http://dx.doi.org/10.1080/20961790.2019.1665160 Text en © 2019 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Tao, Ruiyang
Chen, Chong
Sheng, Xiang
Xia, Ruocheng
Zhang, Xiaochun
Zhang, Jingyi
Yang, Zihao
Zhang, Suhua
Li, Chengtao
Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
title Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
title_full Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
title_fullStr Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
title_full_unstemmed Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
title_short Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
title_sort validation of the investigator 24plex qs kit: a 6-dye multiplex pcr assay for forensic application in the chinese han population
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246015/
https://www.ncbi.nlm.nih.gov/pubmed/35784410
http://dx.doi.org/10.1080/20961790.2019.1665160
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