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Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard se...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246015/ https://www.ncbi.nlm.nih.gov/pubmed/35784410 http://dx.doi.org/10.1080/20961790.2019.1665160 |
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author | Tao, Ruiyang Chen, Chong Sheng, Xiang Xia, Ruocheng Zhang, Xiaochun Zhang, Jingyi Yang, Zihao Zhang, Suhua Li, Chengtao |
author_facet | Tao, Ruiyang Chen, Chong Sheng, Xiang Xia, Ruocheng Zhang, Xiaochun Zhang, Jingyi Yang, Zihao Zhang, Suhua Li, Chengtao |
author_sort | Tao, Ruiyang |
collection | PubMed |
description | The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population. |
format | Online Article Text |
id | pubmed-9246015 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-92460152022-07-01 Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population Tao, Ruiyang Chen, Chong Sheng, Xiang Xia, Ruocheng Zhang, Xiaochun Zhang, Jingyi Yang, Zihao Zhang, Suhua Li, Chengtao Forensic Sci Res Original Articles The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population. Taylor & Francis 2019-12-06 /pmc/articles/PMC9246015/ /pubmed/35784410 http://dx.doi.org/10.1080/20961790.2019.1665160 Text en © 2019 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Tao, Ruiyang Chen, Chong Sheng, Xiang Xia, Ruocheng Zhang, Xiaochun Zhang, Jingyi Yang, Zihao Zhang, Suhua Li, Chengtao Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
title | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
title_full | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
title_fullStr | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
title_full_unstemmed | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
title_short | Validation of the Investigator 24plex QS Kit: a 6-dye multiplex PCR assay for forensic application in the Chinese Han population |
title_sort | validation of the investigator 24plex qs kit: a 6-dye multiplex pcr assay for forensic application in the chinese han population |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246015/ https://www.ncbi.nlm.nih.gov/pubmed/35784410 http://dx.doi.org/10.1080/20961790.2019.1665160 |
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