Cargando…
Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions
Cystinuria is one of various disorders that cause biomineralization in the urinary system, including bladder stone formation in humans. It is most prevalent in children and adolescents and more aggressive in males. There is no cure, and only limited disease management techniques help to solubilize t...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246204/ https://www.ncbi.nlm.nih.gov/pubmed/35771811 http://dx.doi.org/10.1371/journal.pone.0250137 |
_version_ | 1784738922496000000 |
---|---|
author | Rose, Jacob Basisty, Nathan Zee, Tiffany Wehrfritz, Cameron Bose, Neelanjan Desprez, Pierre-Yves Kapahi, Pankaj Stoller, Marshall Schilling, Birgit |
author_facet | Rose, Jacob Basisty, Nathan Zee, Tiffany Wehrfritz, Cameron Bose, Neelanjan Desprez, Pierre-Yves Kapahi, Pankaj Stoller, Marshall Schilling, Birgit |
author_sort | Rose, Jacob |
collection | PubMed |
description | Cystinuria is one of various disorders that cause biomineralization in the urinary system, including bladder stone formation in humans. It is most prevalent in children and adolescents and more aggressive in males. There is no cure, and only limited disease management techniques help to solubilize the stones. Recurrence, even after treatment, occurs frequently. Other than a buildup of cystine, little is known about factors involved in the formation, expansion, and recurrence of these stones. This study sought to define the growth of bladder stones, guided by micro-computed tomography imaging, and to profile dynamic stone proteome changes in a cystinuria mouse model. After bladder stones developed in vivo, they were harvested and separated into four developmental stages (sand, small, medium and large stone), based on their size. Data-dependent and data-independent acquisitions allowed deep profiling of stone proteomics. The proteomic signatures and pathways illustrated major changes as the stones grew. Stones initiate from a small nidus, grow outward, and show major enrichment in ribosomal proteins and factors related to coagulation and platelet degranulation, suggesting a major dysregulation in specific pathways that can be targeted for new therapeutic options. |
format | Online Article Text |
id | pubmed-9246204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92462042022-07-01 Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions Rose, Jacob Basisty, Nathan Zee, Tiffany Wehrfritz, Cameron Bose, Neelanjan Desprez, Pierre-Yves Kapahi, Pankaj Stoller, Marshall Schilling, Birgit PLoS One Research Article Cystinuria is one of various disorders that cause biomineralization in the urinary system, including bladder stone formation in humans. It is most prevalent in children and adolescents and more aggressive in males. There is no cure, and only limited disease management techniques help to solubilize the stones. Recurrence, even after treatment, occurs frequently. Other than a buildup of cystine, little is known about factors involved in the formation, expansion, and recurrence of these stones. This study sought to define the growth of bladder stones, guided by micro-computed tomography imaging, and to profile dynamic stone proteome changes in a cystinuria mouse model. After bladder stones developed in vivo, they were harvested and separated into four developmental stages (sand, small, medium and large stone), based on their size. Data-dependent and data-independent acquisitions allowed deep profiling of stone proteomics. The proteomic signatures and pathways illustrated major changes as the stones grew. Stones initiate from a small nidus, grow outward, and show major enrichment in ribosomal proteins and factors related to coagulation and platelet degranulation, suggesting a major dysregulation in specific pathways that can be targeted for new therapeutic options. Public Library of Science 2022-06-30 /pmc/articles/PMC9246204/ /pubmed/35771811 http://dx.doi.org/10.1371/journal.pone.0250137 Text en © 2022 Rose et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rose, Jacob Basisty, Nathan Zee, Tiffany Wehrfritz, Cameron Bose, Neelanjan Desprez, Pierre-Yves Kapahi, Pankaj Stoller, Marshall Schilling, Birgit Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions |
title | Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions |
title_full | Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions |
title_fullStr | Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions |
title_full_unstemmed | Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions |
title_short | Comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions |
title_sort | comprehensive proteomic quantification of bladder stone progression in a cystinuric mouse model using data-independent acquisitions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246204/ https://www.ncbi.nlm.nih.gov/pubmed/35771811 http://dx.doi.org/10.1371/journal.pone.0250137 |
work_keys_str_mv | AT rosejacob comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT basistynathan comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT zeetiffany comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT wehrfritzcameron comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT boseneelanjan comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT desprezpierreyves comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT kapahipankaj comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT stollermarshall comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions AT schillingbirgit comprehensiveproteomicquantificationofbladderstoneprogressioninacystinuricmousemodelusingdataindependentacquisitions |