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Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation

Asperosaponin VI (AS6), as the quality marker of Dipsaci Radix, is verified to exert therapeutic effect on alleviating recurrent spontaneous abortion (RSA). However, due to the lack of relevant research, its molecular mechanism is still unclear. We retrieved targets for AS6 and RSA, and then used th...

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Autores principales: Xia, Bo, Zhang, Peng, Lai, Yuling, Cui, Shichao, Chen, Zhenyue, Yu, Qingying, Wu, Haiwang, Zeng, Lihua, Xie, Baozhen, Li, Jingwei, Zhang, Huimin, Luo, Songping, Gao, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246589/
https://www.ncbi.nlm.nih.gov/pubmed/35783512
http://dx.doi.org/10.1155/2022/8099853
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author Xia, Bo
Zhang, Peng
Lai, Yuling
Cui, Shichao
Chen, Zhenyue
Yu, Qingying
Wu, Haiwang
Zeng, Lihua
Xie, Baozhen
Li, Jingwei
Zhang, Huimin
Luo, Songping
Gao, Jie
author_facet Xia, Bo
Zhang, Peng
Lai, Yuling
Cui, Shichao
Chen, Zhenyue
Yu, Qingying
Wu, Haiwang
Zeng, Lihua
Xie, Baozhen
Li, Jingwei
Zhang, Huimin
Luo, Songping
Gao, Jie
author_sort Xia, Bo
collection PubMed
description Asperosaponin VI (AS6), as the quality marker of Dipsaci Radix, is verified to exert therapeutic effect on alleviating recurrent spontaneous abortion (RSA). However, due to the lack of relevant research, its molecular mechanism is still unclear. We retrieved targets for AS6 and RSA, and then used their overlapped targets for PPI analysis. In addition, we used GO and KEGG enrichment analyses, and molecular docking to investigate the anti-RSA mechanisms of AS6. Furthermore, we conducted in vitro experiments to validate the predictions of network pharmacology. Results showed that a total of 103 AS6-associated targets and 2084 RSA-associated targets, with 49 targets overlapped. GO enrichment analysis showed 845 significant biological processes like decidualization, while KEGG pathway enrichment analysis revealed 76 significant entries including 18 signaling pathways, which were closely linked to PI3K-Akt, HIF-1, TNF, IL-17, and VEGF signaling pathways, etc. Molecular docking findings verified that AS6 had tight link with the key targets including JUN, CASP3, STAT3, SRC, and PTGS2. Notably, in vitro experiments revealed that AS6 treatment could exert lower expressions of JUN, pro-CASP3, CASP3, STAT3, SRC, and PTGS2 in decidual cells compared with progesterone despite the expressions of STAT3, SRC, and PTGS2 with no significant difference, and mifepristone could interfere with the effects. In general, numerous targets and multiple pathways involve during the process of AS6 treatment against RSA. Moreover, our in vitro research first reported that AS6 may regulate the expressions of key targets (JUN, CASP3, STAT3, SRC, and PTGS2) in decidual cells to promote decidualization, thus treating RSA.
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spelling pubmed-92465892022-07-01 Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation Xia, Bo Zhang, Peng Lai, Yuling Cui, Shichao Chen, Zhenyue Yu, Qingying Wu, Haiwang Zeng, Lihua Xie, Baozhen Li, Jingwei Zhang, Huimin Luo, Songping Gao, Jie Evid Based Complement Alternat Med Research Article Asperosaponin VI (AS6), as the quality marker of Dipsaci Radix, is verified to exert therapeutic effect on alleviating recurrent spontaneous abortion (RSA). However, due to the lack of relevant research, its molecular mechanism is still unclear. We retrieved targets for AS6 and RSA, and then used their overlapped targets for PPI analysis. In addition, we used GO and KEGG enrichment analyses, and molecular docking to investigate the anti-RSA mechanisms of AS6. Furthermore, we conducted in vitro experiments to validate the predictions of network pharmacology. Results showed that a total of 103 AS6-associated targets and 2084 RSA-associated targets, with 49 targets overlapped. GO enrichment analysis showed 845 significant biological processes like decidualization, while KEGG pathway enrichment analysis revealed 76 significant entries including 18 signaling pathways, which were closely linked to PI3K-Akt, HIF-1, TNF, IL-17, and VEGF signaling pathways, etc. Molecular docking findings verified that AS6 had tight link with the key targets including JUN, CASP3, STAT3, SRC, and PTGS2. Notably, in vitro experiments revealed that AS6 treatment could exert lower expressions of JUN, pro-CASP3, CASP3, STAT3, SRC, and PTGS2 in decidual cells compared with progesterone despite the expressions of STAT3, SRC, and PTGS2 with no significant difference, and mifepristone could interfere with the effects. In general, numerous targets and multiple pathways involve during the process of AS6 treatment against RSA. Moreover, our in vitro research first reported that AS6 may regulate the expressions of key targets (JUN, CASP3, STAT3, SRC, and PTGS2) in decidual cells to promote decidualization, thus treating RSA. Hindawi 2022-06-23 /pmc/articles/PMC9246589/ /pubmed/35783512 http://dx.doi.org/10.1155/2022/8099853 Text en Copyright © 2022 Bo Xia et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xia, Bo
Zhang, Peng
Lai, Yuling
Cui, Shichao
Chen, Zhenyue
Yu, Qingying
Wu, Haiwang
Zeng, Lihua
Xie, Baozhen
Li, Jingwei
Zhang, Huimin
Luo, Songping
Gao, Jie
Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation
title Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation
title_full Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation
title_fullStr Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation
title_full_unstemmed Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation
title_short Research on the Mechanism of Asperosaponin VI for Treating Recurrent Spontaneous Abortion by Bioinformatics Analysis and Experimental Validation
title_sort research on the mechanism of asperosaponin vi for treating recurrent spontaneous abortion by bioinformatics analysis and experimental validation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246589/
https://www.ncbi.nlm.nih.gov/pubmed/35783512
http://dx.doi.org/10.1155/2022/8099853
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