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Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats

OBJECTIVE: Fibrin sealant (FS) is widely used for skin wound healing, but data on porcine FS (PFS), a new type of FS, are limited. This study investigated the effects and potential mechanisms of porcine fibrin sealant (PFS) on skin wound healing in rats. Methods. Traumatic rats were randomly divided...

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Autores principales: Zhang, Lihuo, Liu, Lu, Zhang, Jundong, Zhou, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246592/
https://www.ncbi.nlm.nih.gov/pubmed/35783522
http://dx.doi.org/10.1155/2022/5063625
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author Zhang, Lihuo
Liu, Lu
Zhang, Jundong
Zhou, Ping
author_facet Zhang, Lihuo
Liu, Lu
Zhang, Jundong
Zhou, Ping
author_sort Zhang, Lihuo
collection PubMed
description OBJECTIVE: Fibrin sealant (FS) is widely used for skin wound healing, but data on porcine FS (PFS), a new type of FS, are limited. This study investigated the effects and potential mechanisms of porcine fibrin sealant (PFS) on skin wound healing in rats. Methods. Traumatic rats were randomly divided into three groups: control, PFS, and medical Vaseline. The wound area and wound index of the rats were measured within 14 days after surgery. Hematoxylin-eosin (H&E) staining and Masson staining were used to observe the pathological images and collagen formation on the wounded skin, respectively. To investigate the healing mechanisms, the enzyme-linked immunosorbent assay (ELISA) was used to detect platelet endothelial cell adhesion molecule-1 (CD31) and cluster of differentiation 34 (CD34) expression in the wounded skin. Additionally, quantitative real-time PCR (qRT-PCR) was used to evaluate the mRNA levels of the vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and epidermal growth factor (EGF), and transforming growth factor-β1 (TGF-β1). Meanwhile, TGF-β1 protein expression was assessed by Western blot analysis. RESULTS: Compared with the control group, both PFS and medical Vaseline treatment significantly reduced the wounded area and increased the wound closure rate. H&E staining showed that the cells in the PFS group proliferated rapidly, and the epidermis and dermis were thickened to some extent with a clear epidermal cell structure. Moreover, PFS promoted the formation of collagen and significantly increased the levels of CD31 and CD34 and the growth factors in the skin tissues of the traumatic rats. CONCLUSION: PFS effectively promoted skin wound healing, especially in tissue formation, reepithelialization, angiogenesis, and collagen deposition, in traumatic rat models. This study provides a new strategy and scientific foundation for PFS application in skin wound healing.
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spelling pubmed-92465922022-07-01 Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats Zhang, Lihuo Liu, Lu Zhang, Jundong Zhou, Ping Evid Based Complement Alternat Med Research Article OBJECTIVE: Fibrin sealant (FS) is widely used for skin wound healing, but data on porcine FS (PFS), a new type of FS, are limited. This study investigated the effects and potential mechanisms of porcine fibrin sealant (PFS) on skin wound healing in rats. Methods. Traumatic rats were randomly divided into three groups: control, PFS, and medical Vaseline. The wound area and wound index of the rats were measured within 14 days after surgery. Hematoxylin-eosin (H&E) staining and Masson staining were used to observe the pathological images and collagen formation on the wounded skin, respectively. To investigate the healing mechanisms, the enzyme-linked immunosorbent assay (ELISA) was used to detect platelet endothelial cell adhesion molecule-1 (CD31) and cluster of differentiation 34 (CD34) expression in the wounded skin. Additionally, quantitative real-time PCR (qRT-PCR) was used to evaluate the mRNA levels of the vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and epidermal growth factor (EGF), and transforming growth factor-β1 (TGF-β1). Meanwhile, TGF-β1 protein expression was assessed by Western blot analysis. RESULTS: Compared with the control group, both PFS and medical Vaseline treatment significantly reduced the wounded area and increased the wound closure rate. H&E staining showed that the cells in the PFS group proliferated rapidly, and the epidermis and dermis were thickened to some extent with a clear epidermal cell structure. Moreover, PFS promoted the formation of collagen and significantly increased the levels of CD31 and CD34 and the growth factors in the skin tissues of the traumatic rats. CONCLUSION: PFS effectively promoted skin wound healing, especially in tissue formation, reepithelialization, angiogenesis, and collagen deposition, in traumatic rat models. This study provides a new strategy and scientific foundation for PFS application in skin wound healing. Hindawi 2022-06-23 /pmc/articles/PMC9246592/ /pubmed/35783522 http://dx.doi.org/10.1155/2022/5063625 Text en Copyright © 2022 Lihuo Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Lihuo
Liu, Lu
Zhang, Jundong
Zhou, Ping
Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats
title Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats
title_full Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats
title_fullStr Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats
title_full_unstemmed Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats
title_short Porcine Fibrin Sealant Promotes Skin Wound Healing in Rats
title_sort porcine fibrin sealant promotes skin wound healing in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246592/
https://www.ncbi.nlm.nih.gov/pubmed/35783522
http://dx.doi.org/10.1155/2022/5063625
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