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Biological features of canine cancer-associated fibroblasts and their influence on cancer cell invasion
Cancer-associated fibroblasts (CAFs) play an essential role in tumor invasion and metastasis. In dogs, the biological features of CAFs have not been well characterized. The purpose of this study was to investigate differences in the biological activities of canine CAFs and normal fibroblasts (NFs),...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Japanese Society of Veterinary Science
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246690/ https://www.ncbi.nlm.nih.gov/pubmed/35400675 http://dx.doi.org/10.1292/jvms.22-0041 |
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author | KUDO, Ayano YOSHIMOTO, Sho YOSHIDA, Hiromitsu IZUMI, Yusuke TAKAGI, Satoshi |
author_facet | KUDO, Ayano YOSHIMOTO, Sho YOSHIDA, Hiromitsu IZUMI, Yusuke TAKAGI, Satoshi |
author_sort | KUDO, Ayano |
collection | PubMed |
description | Cancer-associated fibroblasts (CAFs) play an essential role in tumor invasion and metastasis. In dogs, the biological features of CAFs have not been well characterized. The purpose of this study was to investigate differences in the biological activities of canine CAFs and normal fibroblasts (NFs), and their influence on the migration and invasion of cancer cells. Canine CAFs and NFs were harvested from surgically-resected malignant epithelial tumor tissues and skin tissues of dogs. A wound-healing assay was conducted to compare the migratory and invasive abilities of canine CAFs and NFs. The results of this study showed that canine CAFs have a greater migratory and invasive ability than NFs. To observe the indirect and direct interactions between fibroblasts and cancer cells, Boyden chamber assay and 3D co-culture with collagen gel were conducted. The number of migrated and infiltrated cancer cells co-cultured with canine CAFs was greater than that with NFs. In the 3D co-culture, cancer cells showed noteworthy proliferation on the surface of gels containing canine CAFs and invasion into the gel. On the other hand, no infiltration of cancer cells into the gel containing NFs was observed. It was suggested that canine CAFs activate migration and invasion of cancer cells and promote the infiltration of cancer cells into collagen gels. |
format | Online Article Text |
id | pubmed-9246690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-92466902022-07-09 Biological features of canine cancer-associated fibroblasts and their influence on cancer cell invasion KUDO, Ayano YOSHIMOTO, Sho YOSHIDA, Hiromitsu IZUMI, Yusuke TAKAGI, Satoshi J Vet Med Sci Surgery Cancer-associated fibroblasts (CAFs) play an essential role in tumor invasion and metastasis. In dogs, the biological features of CAFs have not been well characterized. The purpose of this study was to investigate differences in the biological activities of canine CAFs and normal fibroblasts (NFs), and their influence on the migration and invasion of cancer cells. Canine CAFs and NFs were harvested from surgically-resected malignant epithelial tumor tissues and skin tissues of dogs. A wound-healing assay was conducted to compare the migratory and invasive abilities of canine CAFs and NFs. The results of this study showed that canine CAFs have a greater migratory and invasive ability than NFs. To observe the indirect and direct interactions between fibroblasts and cancer cells, Boyden chamber assay and 3D co-culture with collagen gel were conducted. The number of migrated and infiltrated cancer cells co-cultured with canine CAFs was greater than that with NFs. In the 3D co-culture, cancer cells showed noteworthy proliferation on the surface of gels containing canine CAFs and invasion into the gel. On the other hand, no infiltration of cancer cells into the gel containing NFs was observed. It was suggested that canine CAFs activate migration and invasion of cancer cells and promote the infiltration of cancer cells into collagen gels. The Japanese Society of Veterinary Science 2022-04-11 2022-06 /pmc/articles/PMC9246690/ /pubmed/35400675 http://dx.doi.org/10.1292/jvms.22-0041 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Surgery KUDO, Ayano YOSHIMOTO, Sho YOSHIDA, Hiromitsu IZUMI, Yusuke TAKAGI, Satoshi Biological features of canine cancer-associated fibroblasts and their influence on cancer cell invasion |
title | Biological features of canine cancer-associated fibroblasts and their
influence on cancer cell invasion |
title_full | Biological features of canine cancer-associated fibroblasts and their
influence on cancer cell invasion |
title_fullStr | Biological features of canine cancer-associated fibroblasts and their
influence on cancer cell invasion |
title_full_unstemmed | Biological features of canine cancer-associated fibroblasts and their
influence on cancer cell invasion |
title_short | Biological features of canine cancer-associated fibroblasts and their
influence on cancer cell invasion |
title_sort | biological features of canine cancer-associated fibroblasts and their
influence on cancer cell invasion |
topic | Surgery |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246690/ https://www.ncbi.nlm.nih.gov/pubmed/35400675 http://dx.doi.org/10.1292/jvms.22-0041 |
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