A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA

A cell line (PL38PB) was established from blood samples of a 6-month-old pig that was diagnosed with lymphoma with CD5 expression. Histopathological examination revealed neoplastic lesions in the spleen, liver and lymph nodes. Tumor cells were immunohistochemically positive for CD20 and immunoglobul...

Descripción completa

Detalles Bibliográficos
Autores principales: OGIHARA, Kikumi, NAYA, Yuko, KAMIE, Junichi, HISAMATSU, Shin, KODAMA, Michi, ISHIKAWA, Yoshiharu, KADOTA, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2022
Materias:
Pathology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246694/
https://www.ncbi.nlm.nih.gov/pubmed/35400671
http://dx.doi.org/10.1292/jvms.21-0508
_version_ 1784739020176097280
author OGIHARA, Kikumi
NAYA, Yuko
KAMIE, Junichi
HISAMATSU, Shin
KODAMA, Michi
ISHIKAWA, Yoshiharu
KADOTA, Koichi
author_facet OGIHARA, Kikumi
NAYA, Yuko
KAMIE, Junichi
HISAMATSU, Shin
KODAMA, Michi
ISHIKAWA, Yoshiharu
KADOTA, Koichi
author_sort OGIHARA, Kikumi
collection PubMed
description A cell line (PL38PB) was established from blood samples of a 6-month-old pig that was diagnosed with lymphoma with CD5 expression. Histopathological examination revealed neoplastic lesions in the spleen, liver and lymph nodes. Tumor cells were immunohistochemically positive for CD20 and immunoglobulin heavy chains (μ, γ and α). Membranous CD5 and cytoplasmic Immunoglobulin M (IgM), ​Immunoglobulin G (IgG) and ​Immunoglobulin A (IgA) were detected in PL38PB cells by flow cytometry. In addition, the cytoplasm of PL38PB cells were positive for IgM, IgG and IgA by immunofluorescent. However, no Ig secretion was detected in culture supernatant by Ouchterlony gel diffusion method. Results suggest that PL38PB cells express three Ig isotypes that are produced but not secreted.
format Online
Article
Text
id pubmed-9246694
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-92466942022-07-09 A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA OGIHARA, Kikumi NAYA, Yuko KAMIE, Junichi HISAMATSU, Shin KODAMA, Michi ISHIKAWA, Yoshiharu KADOTA, Koichi J Vet Med Sci Pathology A cell line (PL38PB) was established from blood samples of a 6-month-old pig that was diagnosed with lymphoma with CD5 expression. Histopathological examination revealed neoplastic lesions in the spleen, liver and lymph nodes. Tumor cells were immunohistochemically positive for CD20 and immunoglobulin heavy chains (μ, γ and α). Membranous CD5 and cytoplasmic Immunoglobulin M (IgM), ​Immunoglobulin G (IgG) and ​Immunoglobulin A (IgA) were detected in PL38PB cells by flow cytometry. In addition, the cytoplasm of PL38PB cells were positive for IgM, IgG and IgA by immunofluorescent. However, no Ig secretion was detected in culture supernatant by Ouchterlony gel diffusion method. Results suggest that PL38PB cells express three Ig isotypes that are produced but not secreted. The Japanese Society of Veterinary Science 2022-04-11 2022-06 /pmc/articles/PMC9246694/ /pubmed/35400671 http://dx.doi.org/10.1292/jvms.21-0508 Text en ©2022 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Pathology
OGIHARA, Kikumi
NAYA, Yuko
KAMIE, Junichi
HISAMATSU, Shin
KODAMA, Michi
ISHIKAWA, Yoshiharu
KADOTA, Koichi
A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA
title A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA
title_full A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA
title_fullStr A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA
title_full_unstemmed A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA
title_short A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA
title_sort porcine lymphoma-derived cell line co-expressing igm, igg and iga
topic Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9246694/
https://www.ncbi.nlm.nih.gov/pubmed/35400671
http://dx.doi.org/10.1292/jvms.21-0508
work_keys_str_mv AT ogiharakikumi aporcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT nayayuko aporcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT kamiejunichi aporcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT hisamatsushin aporcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT kodamamichi aporcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT ishikawayoshiharu aporcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT kadotakoichi aporcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT ogiharakikumi porcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT nayayuko porcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT kamiejunichi porcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT hisamatsushin porcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT kodamamichi porcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT ishikawayoshiharu porcinelymphomaderivedcelllinecoexpressingigmiggandiga
AT kadotakoichi porcinelymphomaderivedcelllinecoexpressingigmiggandiga

Ejemplares similares