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First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review

Bacteremia caused by Herbaspirillum huttiense (H. huttiense) is relatively rare in positive blood cultures. H. huttiense is an opportunistic bacterium in patients with cancer and cirrhosis and has also been described in immunocompromised hosts. In this study, H. huttiense was isolated from a patient...

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Autores principales: Li, Xiangyun, Bao, Xundi, Qiao, Guanhua, Wang, Lianzi, Shi, Cuixiao, Chen, Shuyi, Xu, Yuanhong, Zheng, Meijuan, Wang, Zhongxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247281/
https://www.ncbi.nlm.nih.gov/pubmed/35782140
http://dx.doi.org/10.3389/fcimb.2022.882827
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author Li, Xiangyun
Bao, Xundi
Qiao, Guanhua
Wang, Lianzi
Shi, Cuixiao
Chen, Shuyi
Xu, Yuanhong
Zheng, Meijuan
Wang, Zhongxin
author_facet Li, Xiangyun
Bao, Xundi
Qiao, Guanhua
Wang, Lianzi
Shi, Cuixiao
Chen, Shuyi
Xu, Yuanhong
Zheng, Meijuan
Wang, Zhongxin
author_sort Li, Xiangyun
collection PubMed
description Bacteremia caused by Herbaspirillum huttiense (H. huttiense) is relatively rare in positive blood cultures. H. huttiense is an opportunistic bacterium in patients with cancer and cirrhosis and has also been described in immunocompromised hosts. In this study, H. huttiense was isolated from a patient with repeated chest tightness and chest pain. Smears were prepared, stained, and examined by microscopy. Single colonies were analyzed by Gram staining, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), 16S rRNA sequencing and Next-Generation Sequencing (NGS). Antibiotic sensitivity was assessed by agar dilution. Almost all publications on H. huttiense infections in the PubMed/ScienceDirect/EBSCO databases were reviewed and summarized. Blood sample culturing yielded white, gelatinous, and slightly raised colonies without hemolytic rings. The bacilli were found to be Gram-negative, and MS results showed 99.2% homology with H. huttiense. This was confirmed by 16S rRNA gene sequencing, phylogenetic tree analysis and NGS all of which were homologous with H. huttiense in GenBank. Antibiotic susceptibility tests were performed to determine the minimum inhibitory concentrations (MICs) of imipenem, meropenem, piperacillin-tazobactam, and levofloxacin. A comprehensive literature review revealed that H. huttiense was an emergent pathogen. After medical treatment, the patient’s body temperature returned to normal. This is the first report of bacteremia caused by H. huttiense in China. The findings could improve the awareness and attention of the rare pathogenic microorganisms in China.
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spelling pubmed-92472812022-07-02 First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review Li, Xiangyun Bao, Xundi Qiao, Guanhua Wang, Lianzi Shi, Cuixiao Chen, Shuyi Xu, Yuanhong Zheng, Meijuan Wang, Zhongxin Front Cell Infect Microbiol Cellular and Infection Microbiology Bacteremia caused by Herbaspirillum huttiense (H. huttiense) is relatively rare in positive blood cultures. H. huttiense is an opportunistic bacterium in patients with cancer and cirrhosis and has also been described in immunocompromised hosts. In this study, H. huttiense was isolated from a patient with repeated chest tightness and chest pain. Smears were prepared, stained, and examined by microscopy. Single colonies were analyzed by Gram staining, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS), 16S rRNA sequencing and Next-Generation Sequencing (NGS). Antibiotic sensitivity was assessed by agar dilution. Almost all publications on H. huttiense infections in the PubMed/ScienceDirect/EBSCO databases were reviewed and summarized. Blood sample culturing yielded white, gelatinous, and slightly raised colonies without hemolytic rings. The bacilli were found to be Gram-negative, and MS results showed 99.2% homology with H. huttiense. This was confirmed by 16S rRNA gene sequencing, phylogenetic tree analysis and NGS all of which were homologous with H. huttiense in GenBank. Antibiotic susceptibility tests were performed to determine the minimum inhibitory concentrations (MICs) of imipenem, meropenem, piperacillin-tazobactam, and levofloxacin. A comprehensive literature review revealed that H. huttiense was an emergent pathogen. After medical treatment, the patient’s body temperature returned to normal. This is the first report of bacteremia caused by H. huttiense in China. The findings could improve the awareness and attention of the rare pathogenic microorganisms in China. Frontiers Media S.A. 2022-06-17 /pmc/articles/PMC9247281/ /pubmed/35782140 http://dx.doi.org/10.3389/fcimb.2022.882827 Text en Copyright © 2022 Li, Bao, Qiao, Wang, Shi, Chen, Xu, Zheng and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Xiangyun
Bao, Xundi
Qiao, Guanhua
Wang, Lianzi
Shi, Cuixiao
Chen, Shuyi
Xu, Yuanhong
Zheng, Meijuan
Wang, Zhongxin
First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review
title First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review
title_full First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review
title_fullStr First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review
title_full_unstemmed First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review
title_short First Study of Bacteremia Caused by Herbaspirillum huttiense in China: A Brief Research Report and Literature Review
title_sort first study of bacteremia caused by herbaspirillum huttiense in china: a brief research report and literature review
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247281/
https://www.ncbi.nlm.nih.gov/pubmed/35782140
http://dx.doi.org/10.3389/fcimb.2022.882827
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