Cargando…

Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro

Networks of neurons are typically studied in the field of Criticality. However, the study of astrocyte networks in the brain has been recently lauded to be of equal importance to that of the neural networks. To date criticality assessments have only been performed on networks astrocytes from healthy...

Descripción completa

Detalles Bibliográficos
Autores principales: Mellor, Nicholas G., Graham, E. Scott, Unsworth, Charles P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247335/
https://www.ncbi.nlm.nih.gov/pubmed/35784870
http://dx.doi.org/10.3389/fphys.2022.808730
_version_ 1784739143334494208
author Mellor, Nicholas G.
Graham, E. Scott
Unsworth, Charles P.
author_facet Mellor, Nicholas G.
Graham, E. Scott
Unsworth, Charles P.
author_sort Mellor, Nicholas G.
collection PubMed
description Networks of neurons are typically studied in the field of Criticality. However, the study of astrocyte networks in the brain has been recently lauded to be of equal importance to that of the neural networks. To date criticality assessments have only been performed on networks astrocytes from healthy rats, and astrocytes from cultured dissociated resections of intractable epilepsy. This work, for the first time, presents studies of the critical dynamics and shape collapse of calcium waves observed in cultures of healthy human astrocyte networks in vitro, derived from the human hNT cell line. In this article, we demonstrate that avalanches of spontaneous calcium waves display strong critical dynamics, including power-laws in both the size and duration distributions. In addition, the temporal profiles of avalanches displayed self-similarity, leading to shape collapse of the temporal profiles. These findings are significant as they suggest that cultured networks of healthy human hNT astrocytes self-organize to a critical point, implying that healthy astrocytic networks operate at a critical point to process and transmit information. Furthermore, this work can serve as a point of reference to which other astrocyte criticality studies can be compared.
format Online
Article
Text
id pubmed-9247335
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92473352022-07-02 Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro Mellor, Nicholas G. Graham, E. Scott Unsworth, Charles P. Front Physiol Physiology Networks of neurons are typically studied in the field of Criticality. However, the study of astrocyte networks in the brain has been recently lauded to be of equal importance to that of the neural networks. To date criticality assessments have only been performed on networks astrocytes from healthy rats, and astrocytes from cultured dissociated resections of intractable epilepsy. This work, for the first time, presents studies of the critical dynamics and shape collapse of calcium waves observed in cultures of healthy human astrocyte networks in vitro, derived from the human hNT cell line. In this article, we demonstrate that avalanches of spontaneous calcium waves display strong critical dynamics, including power-laws in both the size and duration distributions. In addition, the temporal profiles of avalanches displayed self-similarity, leading to shape collapse of the temporal profiles. These findings are significant as they suggest that cultured networks of healthy human hNT astrocytes self-organize to a critical point, implying that healthy astrocytic networks operate at a critical point to process and transmit information. Furthermore, this work can serve as a point of reference to which other astrocyte criticality studies can be compared. Frontiers Media S.A. 2022-06-17 /pmc/articles/PMC9247335/ /pubmed/35784870 http://dx.doi.org/10.3389/fphys.2022.808730 Text en Copyright © 2022 Mellor, Graham and Unsworth. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Mellor, Nicholas G.
Graham, E. Scott
Unsworth, Charles P.
Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro
title Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro
title_full Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro
title_fullStr Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro
title_full_unstemmed Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro
title_short Critical Spatial-Temporal Dynamics and Prominent Shape Collapse of Calcium Waves Observed in Human hNT Astrocytes in Vitro
title_sort critical spatial-temporal dynamics and prominent shape collapse of calcium waves observed in human hnt astrocytes in vitro
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247335/
https://www.ncbi.nlm.nih.gov/pubmed/35784870
http://dx.doi.org/10.3389/fphys.2022.808730
work_keys_str_mv AT mellornicholasg criticalspatialtemporaldynamicsandprominentshapecollapseofcalciumwavesobservedinhumanhntastrocytesinvitro
AT grahamescott criticalspatialtemporaldynamicsandprominentshapecollapseofcalciumwavesobservedinhumanhntastrocytesinvitro
AT unsworthcharlesp criticalspatialtemporaldynamicsandprominentshapecollapseofcalciumwavesobservedinhumanhntastrocytesinvitro