Epidemiology of bla(CTX-M)-Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010–2017

Salmonella enterica can lead to intestinal diarrhea, and the emergence and spread of cephalosporin-resistant Salmonella have brought great challenges to clinical treatment. Therefore, this study investigated the prevalence and transmission of bla(CTX-M) genes among S. Typhimurium from diarrhoeal out...

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Detalles Bibliográficos
Autores principales: Jiang, Qi, Ke, Bi-xia, Wu, De-shu, Wang, Dong, Fang, Liang-xing, Sun, Ruan-yang, Wang, Min-ge, Lei, Jing-er, Shao, Zheng, Liao, Xiao-ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247517/
https://www.ncbi.nlm.nih.gov/pubmed/35783425
http://dx.doi.org/10.3389/fmicb.2022.865254
Descripción
Sumario:Salmonella enterica can lead to intestinal diarrhea, and the emergence and spread of cephalosporin-resistant Salmonella have brought great challenges to clinical treatment. Therefore, this study investigated the prevalence and transmission of bla(CTX-M) genes among S. Typhimurium from diarrhoeal outpatients in Guangdong, China, from 2010 to 2017. A total of 221 bla(CTX-M)-positive isolates were recovered from 1,263 S. Typhimurium isolates from the facal samples of diarrhoea patients in 45 general hospitals from 11 cities. The most popular CTX-M gene was bla(CTX-M-55) (39.6%, 72/182) in the CTX-M-1 group, followed by bla(CTX-M-14) (22.5%, 41/182) and bla(CTX-M-65) (19.2%, 35/182) in the CTX-M-9 group. The isolates that carried bla(CTX-M-9G) had significantly higher resistance rates to multiple antibacterials compared with bla(CTX-M-1G) (p < 0.01). Meanwhile, PFGE analysis not only showed the clonal transmission of bla(CTX-M-55/14/65)-positve isolates of diarrhoeal outpatients’ origins from different hospitals in Guangdong province, but also the characteristic of bla(CTX-M-55/14/65)-positve isolates’ bacterial persistence. Multilocus sequence typing (MLST) analysis indicated that these S. Typhimurium isolates possessed ST34 and ST19. Furthermore, genomic Beast phylogenomic analysis provided the evidence of a close relationship of bla(CTX-M)-positive S. Typhimurium isolates between the outpatients and pork. Most bla(CTX-M-55/14/65) genes were transmitted by non-typeable or IncI1/IncFII/IncHI2 plasmids with the size of ranging from ~80 to ~280 kb. Moreover, whole-genome sequencing (WGS) analysis further revealed that bla(CTX-M-55/14/65) coexisted with other 25 types of ARGs, of which 11 ARGs were highly prevalent with the detection rates >50%, and it first reported the emergence of bla(TEM-141) in S. Typhimurium. This study underscores the importance of surveillance for bla(CTX-M)-positive microbes in diarrhea patients.

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