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Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production

Rhodotorula glutinis, as a member of the family Sporidiobolaceae, is of great value in the field of biotechnology. However, the evolutionary relationship of R. glutinis X-20 with Rhodosporidiobolus, Sporobolomyces, and Rhodotorula are not well understood, and its metabolic pathways such as carotenoi...

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Detalles Bibliográficos
Autores principales: Bo, Shaobo, Ni, Xiaoxia, Guo, Jintang, Liu, Zhengyang, Wang, Xiaoya, Sheng, Yue, Zhang, Genlin, Yang, Jinfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247606/
https://www.ncbi.nlm.nih.gov/pubmed/35782944
http://dx.doi.org/10.3389/fnut.2022.918240
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author Bo, Shaobo
Ni, Xiaoxia
Guo, Jintang
Liu, Zhengyang
Wang, Xiaoya
Sheng, Yue
Zhang, Genlin
Yang, Jinfeng
author_facet Bo, Shaobo
Ni, Xiaoxia
Guo, Jintang
Liu, Zhengyang
Wang, Xiaoya
Sheng, Yue
Zhang, Genlin
Yang, Jinfeng
author_sort Bo, Shaobo
collection PubMed
description Rhodotorula glutinis, as a member of the family Sporidiobolaceae, is of great value in the field of biotechnology. However, the evolutionary relationship of R. glutinis X-20 with Rhodosporidiobolus, Sporobolomyces, and Rhodotorula are not well understood, and its metabolic pathways such as carotenoid biosynthesis are not well resolved. Here, genome sequencing and comparative genome techniques were employed to improve the understanding of R. glutinis X-20. Phytoene desaturase (crtI) and 15-cis-phytoene synthase/lycopene beta-cyclase (crtYB), key enzymes in carotenoid pathway from R. glutinis X-20 were more efficiently expressed in S. cerevisiae INVSc1 than in S. cerevisiae CEN.PK2-1C. High yielding engineered strains were obtained by using synthetic biology technology constructing carotenoid pathway in S. cerevisiae and optimizing the precursor supply after fed-batch fermentation with palmitic acid supplementation. Genome sequencing analysis and metabolite identification has enhanced the understanding of evolutionary relationships and metabolic pathways in R. glutinis X-20, while heterologous construction of carotenoid pathway has facilitated its industrial application.
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spelling pubmed-92476062022-07-02 Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production Bo, Shaobo Ni, Xiaoxia Guo, Jintang Liu, Zhengyang Wang, Xiaoya Sheng, Yue Zhang, Genlin Yang, Jinfeng Front Nutr Nutrition Rhodotorula glutinis, as a member of the family Sporidiobolaceae, is of great value in the field of biotechnology. However, the evolutionary relationship of R. glutinis X-20 with Rhodosporidiobolus, Sporobolomyces, and Rhodotorula are not well understood, and its metabolic pathways such as carotenoid biosynthesis are not well resolved. Here, genome sequencing and comparative genome techniques were employed to improve the understanding of R. glutinis X-20. Phytoene desaturase (crtI) and 15-cis-phytoene synthase/lycopene beta-cyclase (crtYB), key enzymes in carotenoid pathway from R. glutinis X-20 were more efficiently expressed in S. cerevisiae INVSc1 than in S. cerevisiae CEN.PK2-1C. High yielding engineered strains were obtained by using synthetic biology technology constructing carotenoid pathway in S. cerevisiae and optimizing the precursor supply after fed-batch fermentation with palmitic acid supplementation. Genome sequencing analysis and metabolite identification has enhanced the understanding of evolutionary relationships and metabolic pathways in R. glutinis X-20, while heterologous construction of carotenoid pathway has facilitated its industrial application. Frontiers Media S.A. 2022-06-17 /pmc/articles/PMC9247606/ /pubmed/35782944 http://dx.doi.org/10.3389/fnut.2022.918240 Text en Copyright © 2022 Bo, Ni, Guo, Liu, Wang, Sheng, Zhang and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Bo, Shaobo
Ni, Xiaoxia
Guo, Jintang
Liu, Zhengyang
Wang, Xiaoya
Sheng, Yue
Zhang, Genlin
Yang, Jinfeng
Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production
title Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production
title_full Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production
title_fullStr Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production
title_full_unstemmed Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production
title_short Carotenoid Biosynthesis: Genome-Wide Profiling, Pathway Identification in Rhodotorula glutinis X-20, and High-Level Production
title_sort carotenoid biosynthesis: genome-wide profiling, pathway identification in rhodotorula glutinis x-20, and high-level production
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247606/
https://www.ncbi.nlm.nih.gov/pubmed/35782944
http://dx.doi.org/10.3389/fnut.2022.918240
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