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Local aldosterone synthesis in the large intestine of mouse: An ex vivo incubation study
OBJECTIVE: To investigate the regulation of local aldosterone synthesis by physiological stimulants in the murine gut. METHODS: Male mice were fed for 14 days with normal, high (1.6%) or low (0.01%) sodium diets. Tissue liver receptor homolog-1 and aldosterone in the colon and caecum were detected u...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248050/ https://www.ncbi.nlm.nih.gov/pubmed/35748030 http://dx.doi.org/10.1177/03000605221105163 |
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author | Pang, Zan Launonen, Hanna Korpela, Riitta Vapaatalo, Heikki |
author_facet | Pang, Zan Launonen, Hanna Korpela, Riitta Vapaatalo, Heikki |
author_sort | Pang, Zan |
collection | PubMed |
description | OBJECTIVE: To investigate the regulation of local aldosterone synthesis by physiological stimulants in the murine gut. METHODS: Male mice were fed for 14 days with normal, high (1.6%) or low (0.01%) sodium diets. Tissue liver receptor homolog-1 and aldosterone in the colon and caecum were detected using an enzyme-linked immunosorbent assay (ELISA). Released corticosterone and aldosterone in tissue incubation experiments after stimulation with angiotensin II (Ang II) and dibutyryl-cAMP (DBA; the second messenger of adrenocorticotropic hormone) were assayed using an ELISA. Tissue aldosterone synthase (CYP11B2) protein levels were measured using an ELISA and Western blots. RESULTS: In incubated colon tissues, aldosterone synthase levels were increased by a low-sodium diet; and by Ang II and DBA in the normal diet group. Release of aldosterone into the incubation buffer was increased from the colon by a low-sodium diet and decreased by a high-sodium diet in parallel with changes in aldosterone synthase levels. In mice fed a normal diet, colon incubation with both Ang II and DBA increased the release of aldosterone as well as its precursor corticosterone. CONCLUSION: Local aldosterone synthesis in the large intestine is stimulated by a low-sodium diet, dibutyryl-cAMP and Ang II similar to the adrenal glands. |
format | Online Article Text |
id | pubmed-9248050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-92480502022-07-02 Local aldosterone synthesis in the large intestine of mouse: An ex vivo incubation study Pang, Zan Launonen, Hanna Korpela, Riitta Vapaatalo, Heikki J Int Med Res Pre-Clinical Research Report OBJECTIVE: To investigate the regulation of local aldosterone synthesis by physiological stimulants in the murine gut. METHODS: Male mice were fed for 14 days with normal, high (1.6%) or low (0.01%) sodium diets. Tissue liver receptor homolog-1 and aldosterone in the colon and caecum were detected using an enzyme-linked immunosorbent assay (ELISA). Released corticosterone and aldosterone in tissue incubation experiments after stimulation with angiotensin II (Ang II) and dibutyryl-cAMP (DBA; the second messenger of adrenocorticotropic hormone) were assayed using an ELISA. Tissue aldosterone synthase (CYP11B2) protein levels were measured using an ELISA and Western blots. RESULTS: In incubated colon tissues, aldosterone synthase levels were increased by a low-sodium diet; and by Ang II and DBA in the normal diet group. Release of aldosterone into the incubation buffer was increased from the colon by a low-sodium diet and decreased by a high-sodium diet in parallel with changes in aldosterone synthase levels. In mice fed a normal diet, colon incubation with both Ang II and DBA increased the release of aldosterone as well as its precursor corticosterone. CONCLUSION: Local aldosterone synthesis in the large intestine is stimulated by a low-sodium diet, dibutyryl-cAMP and Ang II similar to the adrenal glands. SAGE Publications 2022-06-23 /pmc/articles/PMC9248050/ /pubmed/35748030 http://dx.doi.org/10.1177/03000605221105163 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Report Pang, Zan Launonen, Hanna Korpela, Riitta Vapaatalo, Heikki Local aldosterone synthesis in the large intestine of mouse: An ex vivo incubation study |
title | Local aldosterone synthesis in the large intestine of mouse: An
ex vivo incubation study |
title_full | Local aldosterone synthesis in the large intestine of mouse: An
ex vivo incubation study |
title_fullStr | Local aldosterone synthesis in the large intestine of mouse: An
ex vivo incubation study |
title_full_unstemmed | Local aldosterone synthesis in the large intestine of mouse: An
ex vivo incubation study |
title_short | Local aldosterone synthesis in the large intestine of mouse: An
ex vivo incubation study |
title_sort | local aldosterone synthesis in the large intestine of mouse: an
ex vivo incubation study |
topic | Pre-Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248050/ https://www.ncbi.nlm.nih.gov/pubmed/35748030 http://dx.doi.org/10.1177/03000605221105163 |
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