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An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates
Development of tooth shape is regulated by the enamel knot signalling centre, at least in mammals. Fgf signalling regulates differential proliferation between the enamel knot and adjacent dental epithelia during tooth development, leading to formation of the dental cusp. The presence of an enamel kn...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249395/ https://www.ncbi.nlm.nih.gov/pubmed/35536602 http://dx.doi.org/10.7554/eLife.73173 |
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author | Thiery, Alexandre P Standing, Ariane SI Cooper, Rory L Fraser, Gareth J |
author_facet | Thiery, Alexandre P Standing, Ariane SI Cooper, Rory L Fraser, Gareth J |
author_sort | Thiery, Alexandre P |
collection | PubMed |
description | Development of tooth shape is regulated by the enamel knot signalling centre, at least in mammals. Fgf signalling regulates differential proliferation between the enamel knot and adjacent dental epithelia during tooth development, leading to formation of the dental cusp. The presence of an enamel knot in non-mammalian vertebrates is debated given differences in signalling. Here, we show the conservation and restriction of fgf3, fgf10, and shh to the sites of future dental cusps in the shark (Scyliorhinus canicula), whilst also highlighting striking differences between the shark and mouse. We reveal shifts in tooth size, shape, and cusp number following small molecule perturbations of canonical Wnt signalling. Resulting tooth phenotypes mirror observed effects in mammals, where canonical Wnt has been implicated as an upstream regulator of enamel knot signalling. In silico modelling of shark dental morphogenesis demonstrates how subtle changes in activatory and inhibitory signals can alter tooth shape, resembling developmental phenotypes and cusp shapes observed following experimental Wnt perturbation. Our results support the functional conservation of an enamel knot-like signalling centre throughout vertebrates and suggest that varied tooth types from sharks to mammals follow a similar developmental bauplan. Lineage-specific differences in signalling are not sufficient in refuting homology of this signalling centre, which is likely older than teeth themselves. |
format | Online Article Text |
id | pubmed-9249395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-92493952022-07-02 An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates Thiery, Alexandre P Standing, Ariane SI Cooper, Rory L Fraser, Gareth J eLife Developmental Biology Development of tooth shape is regulated by the enamel knot signalling centre, at least in mammals. Fgf signalling regulates differential proliferation between the enamel knot and adjacent dental epithelia during tooth development, leading to formation of the dental cusp. The presence of an enamel knot in non-mammalian vertebrates is debated given differences in signalling. Here, we show the conservation and restriction of fgf3, fgf10, and shh to the sites of future dental cusps in the shark (Scyliorhinus canicula), whilst also highlighting striking differences between the shark and mouse. We reveal shifts in tooth size, shape, and cusp number following small molecule perturbations of canonical Wnt signalling. Resulting tooth phenotypes mirror observed effects in mammals, where canonical Wnt has been implicated as an upstream regulator of enamel knot signalling. In silico modelling of shark dental morphogenesis demonstrates how subtle changes in activatory and inhibitory signals can alter tooth shape, resembling developmental phenotypes and cusp shapes observed following experimental Wnt perturbation. Our results support the functional conservation of an enamel knot-like signalling centre throughout vertebrates and suggest that varied tooth types from sharks to mammals follow a similar developmental bauplan. Lineage-specific differences in signalling are not sufficient in refuting homology of this signalling centre, which is likely older than teeth themselves. eLife Sciences Publications, Ltd 2022-05-10 /pmc/articles/PMC9249395/ /pubmed/35536602 http://dx.doi.org/10.7554/eLife.73173 Text en © 2022, Thiery et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology Thiery, Alexandre P Standing, Ariane SI Cooper, Rory L Fraser, Gareth J An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates |
title | An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates |
title_full | An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates |
title_fullStr | An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates |
title_full_unstemmed | An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates |
title_short | An epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates |
title_sort | epithelial signalling centre in sharks supports homology of tooth morphogenesis in vertebrates |
topic | Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249395/ https://www.ncbi.nlm.nih.gov/pubmed/35536602 http://dx.doi.org/10.7554/eLife.73173 |
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