Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix

Fluorescence spectroscopy has the potential to identify discriminatory signatures, crucial for early diagnosis of cervical cancer. We demonstrate here the design, fabrication and testing of a 3D printed smartphone based spectroscopic device. Polarized fluorescence and elastic scattering spectra are...

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Autores principales: Shukla, Shivam, Sah, Amar Nath, Hatiboruah, Diganta, Ahirwar, Shikha, Nath, Pabitra, Pradhan, Asima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249735/
https://www.ncbi.nlm.nih.gov/pubmed/35778460
http://dx.doi.org/10.1038/s41598-022-15007-x
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author Shukla, Shivam
Sah, Amar Nath
Hatiboruah, Diganta
Ahirwar, Shikha
Nath, Pabitra
Pradhan, Asima
author_facet Shukla, Shivam
Sah, Amar Nath
Hatiboruah, Diganta
Ahirwar, Shikha
Nath, Pabitra
Pradhan, Asima
author_sort Shukla, Shivam
collection PubMed
description Fluorescence spectroscopy has the potential to identify discriminatory signatures, crucial for early diagnosis of cervical cancer. We demonstrate here the design, fabrication and testing of a 3D printed smartphone based spectroscopic device. Polarized fluorescence and elastic scattering spectra are captured through the device using a 405 nm laser and a white LED source respectively. The device has been calibrated by comparison of spectra of standard fluorophores (Flavin adenine dinucleotide, fluorescein, rhodamine, and porphyrin) with the corresponding spectra collected from a commercial spectrometer. A few cervical tissue spectra have also been captured for proof of its applicability as a portable, standalone device for the collection of intrinsic fluorescence spectra from human cervix.
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spelling pubmed-92497352022-07-03 Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix Shukla, Shivam Sah, Amar Nath Hatiboruah, Diganta Ahirwar, Shikha Nath, Pabitra Pradhan, Asima Sci Rep Article Fluorescence spectroscopy has the potential to identify discriminatory signatures, crucial for early diagnosis of cervical cancer. We demonstrate here the design, fabrication and testing of a 3D printed smartphone based spectroscopic device. Polarized fluorescence and elastic scattering spectra are captured through the device using a 405 nm laser and a white LED source respectively. The device has been calibrated by comparison of spectra of standard fluorophores (Flavin adenine dinucleotide, fluorescein, rhodamine, and porphyrin) with the corresponding spectra collected from a commercial spectrometer. A few cervical tissue spectra have also been captured for proof of its applicability as a portable, standalone device for the collection of intrinsic fluorescence spectra from human cervix. Nature Publishing Group UK 2022-07-01 /pmc/articles/PMC9249735/ /pubmed/35778460 http://dx.doi.org/10.1038/s41598-022-15007-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shukla, Shivam
Sah, Amar Nath
Hatiboruah, Diganta
Ahirwar, Shikha
Nath, Pabitra
Pradhan, Asima
Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix
title Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix
title_full Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix
title_fullStr Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix
title_full_unstemmed Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix
title_short Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix
title_sort design, fabrication and testing of 3d printed smartphone-based device for collection of intrinsic fluorescence from human cervix
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249735/
https://www.ncbi.nlm.nih.gov/pubmed/35778460
http://dx.doi.org/10.1038/s41598-022-15007-x
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