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Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster
The nascent polypeptide-associated complex (NAC) consisting of α- and β-subunits is an essential ribosome-associated protein conserved in eukaryotes. NAC is a ubiquitously expressed co-translational regulator of nascent protein folding and sorting providing for homeostasis of cellular proteins. Here...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249742/ https://www.ncbi.nlm.nih.gov/pubmed/35778515 http://dx.doi.org/10.1038/s41598-022-15233-3 |
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author | Kogan, Galina L. Mikhaleva, Elena A. Olenkina, Oxana M. Ryazansky, Sergei S. Galzitskaya, Oxana V. Abramov, Yuri A. Leinsoo, Toomas A. Akulenko, Natalia V. Lavrov, Sergey A. Gvozdev, Vladimir A. |
author_facet | Kogan, Galina L. Mikhaleva, Elena A. Olenkina, Oxana M. Ryazansky, Sergei S. Galzitskaya, Oxana V. Abramov, Yuri A. Leinsoo, Toomas A. Akulenko, Natalia V. Lavrov, Sergey A. Gvozdev, Vladimir A. |
author_sort | Kogan, Galina L. |
collection | PubMed |
description | The nascent polypeptide-associated complex (NAC) consisting of α- and β-subunits is an essential ribosome-associated protein conserved in eukaryotes. NAC is a ubiquitously expressed co-translational regulator of nascent protein folding and sorting providing for homeostasis of cellular proteins. Here we report on discovering the germline-specific NACαβ paralogs (gNACs), whose β-subunits, non-distinguishable by ordinary immunodetection, are encoded by five highly homologous gene copies, while the α-subunit is encoded by a single αNAC gene. The gNAC expression is detected in the primordial embryonic and adult gonads via immunostaining. The germline-specific α and β subunits differ from the ubiquitously expressed paralogs by the extended intrinsically disordered regions (IDRs) acquired at the N- and C-termini of the coding regions, predicted to be phosphorylated. The presence of distinct phosphorylated isoforms of gNAC-β subunits is confirmed by comparing of their profiles by 2D-isoeletrofocusing resolution before and after phosphatase treatment of testis ribosomes. We revealed that the predicted S/T sites of phosphorylation in the individual orthologous IDRs of gNAC-β sequences of Drosophila species are positionally conserved despite these disordered regions are drastically different. We propose the IDR-dependent molecular crowding and specific coordination of NAC and other proteostasis regulatory factors at the ribosomes of germinal cells. Our findings imply that there may be a functional crosstalk between the germinal and ubiquitous α- and β-subunits based on assessing their depletion effects on the fly viability and gonad development. |
format | Online Article Text |
id | pubmed-9249742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92497422022-07-03 Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster Kogan, Galina L. Mikhaleva, Elena A. Olenkina, Oxana M. Ryazansky, Sergei S. Galzitskaya, Oxana V. Abramov, Yuri A. Leinsoo, Toomas A. Akulenko, Natalia V. Lavrov, Sergey A. Gvozdev, Vladimir A. Sci Rep Article The nascent polypeptide-associated complex (NAC) consisting of α- and β-subunits is an essential ribosome-associated protein conserved in eukaryotes. NAC is a ubiquitously expressed co-translational regulator of nascent protein folding and sorting providing for homeostasis of cellular proteins. Here we report on discovering the germline-specific NACαβ paralogs (gNACs), whose β-subunits, non-distinguishable by ordinary immunodetection, are encoded by five highly homologous gene copies, while the α-subunit is encoded by a single αNAC gene. The gNAC expression is detected in the primordial embryonic and adult gonads via immunostaining. The germline-specific α and β subunits differ from the ubiquitously expressed paralogs by the extended intrinsically disordered regions (IDRs) acquired at the N- and C-termini of the coding regions, predicted to be phosphorylated. The presence of distinct phosphorylated isoforms of gNAC-β subunits is confirmed by comparing of their profiles by 2D-isoeletrofocusing resolution before and after phosphatase treatment of testis ribosomes. We revealed that the predicted S/T sites of phosphorylation in the individual orthologous IDRs of gNAC-β sequences of Drosophila species are positionally conserved despite these disordered regions are drastically different. We propose the IDR-dependent molecular crowding and specific coordination of NAC and other proteostasis regulatory factors at the ribosomes of germinal cells. Our findings imply that there may be a functional crosstalk between the germinal and ubiquitous α- and β-subunits based on assessing their depletion effects on the fly viability and gonad development. Nature Publishing Group UK 2022-07-01 /pmc/articles/PMC9249742/ /pubmed/35778515 http://dx.doi.org/10.1038/s41598-022-15233-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kogan, Galina L. Mikhaleva, Elena A. Olenkina, Oxana M. Ryazansky, Sergei S. Galzitskaya, Oxana V. Abramov, Yuri A. Leinsoo, Toomas A. Akulenko, Natalia V. Lavrov, Sergey A. Gvozdev, Vladimir A. Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster |
title | Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster |
title_full | Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster |
title_fullStr | Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster |
title_full_unstemmed | Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster |
title_short | Extended disordered regions of ribosome-associated NAC proteins paralogs belong only to the germline in Drosophila melanogaster |
title_sort | extended disordered regions of ribosome-associated nac proteins paralogs belong only to the germline in drosophila melanogaster |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249742/ https://www.ncbi.nlm.nih.gov/pubmed/35778515 http://dx.doi.org/10.1038/s41598-022-15233-3 |
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