Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI

Due to limited detection sensitivity and contrast limitation, imaging substrates with (129)Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized (129)Xe MRI. This protocol was optimized f...

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Detalles Bibliográficos
Autores principales: Zeng, Qingbin, Guo, Qianni, Yuan, Yaping, Zhang, Lei, Jiang, Weiping, Yang, Yuqi, Zhou, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249822/
https://www.ncbi.nlm.nih.gov/pubmed/35776640
http://dx.doi.org/10.1016/j.xpro.2022.101499
Descripción
Sumario:Due to limited detection sensitivity and contrast limitation, imaging substrates with (129)Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized (129)Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2021).

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