Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI

Due to limited detection sensitivity and contrast limitation, imaging substrates with (129)Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized (129)Xe MRI. This protocol was optimized f...

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Autores principales: Zeng, Qingbin, Guo, Qianni, Yuan, Yaping, Zhang, Lei, Jiang, Weiping, Yang, Yuqi, Zhou, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249822/
https://www.ncbi.nlm.nih.gov/pubmed/35776640
http://dx.doi.org/10.1016/j.xpro.2022.101499
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author Zeng, Qingbin
Guo, Qianni
Yuan, Yaping
Zhang, Lei
Jiang, Weiping
Yang, Yuqi
Zhou, Xin
author_facet Zeng, Qingbin
Guo, Qianni
Yuan, Yaping
Zhang, Lei
Jiang, Weiping
Yang, Yuqi
Zhou, Xin
author_sort Zeng, Qingbin
collection PubMed
description Due to limited detection sensitivity and contrast limitation, imaging substrates with (129)Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized (129)Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2021).
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spelling pubmed-92498222022-07-03 Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI Zeng, Qingbin Guo, Qianni Yuan, Yaping Zhang, Lei Jiang, Weiping Yang, Yuqi Zhou, Xin STAR Protoc Protocol Due to limited detection sensitivity and contrast limitation, imaging substrates with (129)Xe MRI in living cells is still a challenge. Here, we present an effective protocol to detect and image substrates in human lung cancer cells A549 with hyperpolarized (129)Xe MRI. This protocol was optimized for a cryptophane-based probe sensitive to biothiols and can be expanded to other Xe-based probes to detect potential biomarkers in other mammalian cells. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2021). Elsevier 2022-06-23 /pmc/articles/PMC9249822/ /pubmed/35776640 http://dx.doi.org/10.1016/j.xpro.2022.101499 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zeng, Qingbin
Guo, Qianni
Yuan, Yaping
Zhang, Lei
Jiang, Weiping
Yang, Yuqi
Zhou, Xin
Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI
title Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI
title_full Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI
title_fullStr Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI
title_full_unstemmed Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI
title_short Protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)Xe MRI
title_sort protocol for detecting substrates in living cells by targeted molecular probes through hyperpolarized (129)xe mri
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249822/
https://www.ncbi.nlm.nih.gov/pubmed/35776640
http://dx.doi.org/10.1016/j.xpro.2022.101499
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