Cargando…

Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression

LINC00894 plays an important role in cancer cell proliferation and invasion in breast and kidney cancer. However, its role in thyroid cancer proliferation and metastasis remains unclear. In this study, data on LINC00894 expression in thyroid cancer tissues were obtained from GEPIA2. miRNA expression...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Bo, Liu, Deqing, Chen, Runjie, Guo, Libing, Ran, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249958/
https://www.ncbi.nlm.nih.gov/pubmed/35776220
http://dx.doi.org/10.1007/s12672-022-00520-2
_version_ 1784739703710285824
author Chen, Bo
Liu, Deqing
Chen, Runjie
Guo, Libing
Ran, Jianmin
author_facet Chen, Bo
Liu, Deqing
Chen, Runjie
Guo, Libing
Ran, Jianmin
author_sort Chen, Bo
collection PubMed
description LINC00894 plays an important role in cancer cell proliferation and invasion in breast and kidney cancer. However, its role in thyroid cancer proliferation and metastasis remains unclear. In this study, data on LINC00894 expression in thyroid cancer tissues were obtained from GEPIA2. miRNA expression in thyroid cancer tissues was obtained from starBase 3.0 and OncomiR. Cell proliferation was evaluated using CCK-8, and Transwell chambers were used for the migration and invasion assays. LINC00894 and let-7e-5p expressions in thyroid cancer cells were measured using qRT–PCR. Meanwhile, TIA-1 expression in thyroid cancer cells was analyzed via western blotting. We found that LINC00894 expression was markedly reduced in thyroid cancer tissues and cells, and low expression of LINC00894 was associated with poor prognosis in thyroid cancer. LINC00894 overexpression inhibited the proliferation, migration, and invasion of CAL-62 and TPC-1 cells. Additionally, let-7e-5p expression was substantially enhanced in CAL-62 and TPC-1 cells. LINC00894 overexpression promoted TIA-1 expression by acting as a sponge of let-7e-5p. Finally, let-7e-5p weakened the function of LINC00894 in thyroid cancer cells via reduction in TIA-1 levels. In conclusion, our data suggest that increased LINC00894 expression reduces the oncogenic properties of thyroid cancer cells by sponging let-7e-5p to promote TIA-1 expression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12672-022-00520-2.
format Online
Article
Text
id pubmed-9249958
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-92499582022-07-03 Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression Chen, Bo Liu, Deqing Chen, Runjie Guo, Libing Ran, Jianmin Discov Oncol Research LINC00894 plays an important role in cancer cell proliferation and invasion in breast and kidney cancer. However, its role in thyroid cancer proliferation and metastasis remains unclear. In this study, data on LINC00894 expression in thyroid cancer tissues were obtained from GEPIA2. miRNA expression in thyroid cancer tissues was obtained from starBase 3.0 and OncomiR. Cell proliferation was evaluated using CCK-8, and Transwell chambers were used for the migration and invasion assays. LINC00894 and let-7e-5p expressions in thyroid cancer cells were measured using qRT–PCR. Meanwhile, TIA-1 expression in thyroid cancer cells was analyzed via western blotting. We found that LINC00894 expression was markedly reduced in thyroid cancer tissues and cells, and low expression of LINC00894 was associated with poor prognosis in thyroid cancer. LINC00894 overexpression inhibited the proliferation, migration, and invasion of CAL-62 and TPC-1 cells. Additionally, let-7e-5p expression was substantially enhanced in CAL-62 and TPC-1 cells. LINC00894 overexpression promoted TIA-1 expression by acting as a sponge of let-7e-5p. Finally, let-7e-5p weakened the function of LINC00894 in thyroid cancer cells via reduction in TIA-1 levels. In conclusion, our data suggest that increased LINC00894 expression reduces the oncogenic properties of thyroid cancer cells by sponging let-7e-5p to promote TIA-1 expression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12672-022-00520-2. Springer US 2022-07-01 /pmc/articles/PMC9249958/ /pubmed/35776220 http://dx.doi.org/10.1007/s12672-022-00520-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Chen, Bo
Liu, Deqing
Chen, Runjie
Guo, Libing
Ran, Jianmin
Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression
title Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression
title_full Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression
title_fullStr Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression
title_full_unstemmed Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression
title_short Elevated LINC00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote TIA-1 expression
title_sort elevated linc00894 relieves the oncogenic properties of thyroid cancer cell by sponging let-7e-5p to promote tia-1 expression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249958/
https://www.ncbi.nlm.nih.gov/pubmed/35776220
http://dx.doi.org/10.1007/s12672-022-00520-2
work_keys_str_mv AT chenbo elevatedlinc00894relievestheoncogenicpropertiesofthyroidcancercellbysponginglet7e5ptopromotetia1expression
AT liudeqing elevatedlinc00894relievestheoncogenicpropertiesofthyroidcancercellbysponginglet7e5ptopromotetia1expression
AT chenrunjie elevatedlinc00894relievestheoncogenicpropertiesofthyroidcancercellbysponginglet7e5ptopromotetia1expression
AT guolibing elevatedlinc00894relievestheoncogenicpropertiesofthyroidcancercellbysponginglet7e5ptopromotetia1expression
AT ranjianmin elevatedlinc00894relievestheoncogenicpropertiesofthyroidcancercellbysponginglet7e5ptopromotetia1expression