Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS

The gut microbiota and metabolites play pivotal roles in the pathobiology of various diseases. Here, we describe a protocol to profile the gut microbiome and meta-metabolome of a mouse disease model for acute graft-versus-host disease. We describe steps for fecal sample collection and processing for...

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Detalles Bibliográficos
Autores principales: Li, Xiaoqing, Wu, Peng, Zeng, Xiangjun, Lang, Qiulei, Lin, Yu, Huang, He, Qian, Pengxu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250040/
https://www.ncbi.nlm.nih.gov/pubmed/35776638
http://dx.doi.org/10.1016/j.xpro.2022.101494
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author Li, Xiaoqing
Wu, Peng
Zeng, Xiangjun
Lang, Qiulei
Lin, Yu
Huang, He
Qian, Pengxu
author_facet Li, Xiaoqing
Wu, Peng
Zeng, Xiangjun
Lang, Qiulei
Lin, Yu
Huang, He
Qian, Pengxu
author_sort Li, Xiaoqing
collection PubMed
description The gut microbiota and metabolites play pivotal roles in the pathobiology of various diseases. Here, we describe a protocol to profile the gut microbiome and meta-metabolome of a mouse disease model for acute graft-versus-host disease. We describe steps for fecal sample collection and processing for 16S sequencing and UPLC-MS. Finally, we detail the steps for data analysis and exhibit multi-omic associations to correlate with pathology. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).
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spelling pubmed-92500402022-07-03 Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS Li, Xiaoqing Wu, Peng Zeng, Xiangjun Lang, Qiulei Lin, Yu Huang, He Qian, Pengxu STAR Protoc Protocol The gut microbiota and metabolites play pivotal roles in the pathobiology of various diseases. Here, we describe a protocol to profile the gut microbiome and meta-metabolome of a mouse disease model for acute graft-versus-host disease. We describe steps for fecal sample collection and processing for 16S sequencing and UPLC-MS. Finally, we detail the steps for data analysis and exhibit multi-omic associations to correlate with pathology. For complete details on the use and execution of this protocol, please refer to Li et al. (2020). Elsevier 2022-06-24 /pmc/articles/PMC9250040/ /pubmed/35776638 http://dx.doi.org/10.1016/j.xpro.2022.101494 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Xiaoqing
Wu, Peng
Zeng, Xiangjun
Lang, Qiulei
Lin, Yu
Huang, He
Qian, Pengxu
Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
title Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
title_full Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
title_fullStr Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
title_full_unstemmed Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
title_short Protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16S rDNA sequencing and UPLC-MS
title_sort protocol for correlation analysis of the murine gut microbiome and meta-metabolome using 16s rdna sequencing and uplc-ms
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250040/
https://www.ncbi.nlm.nih.gov/pubmed/35776638
http://dx.doi.org/10.1016/j.xpro.2022.101494
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