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New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma

BACKGROUND: Asthma is a heterogeneous chronic inflammatory disease of the bronchi, the course of which is significantly influenced by extrinsic factors (specific and non‐specific). METHODS: The aim of this study was to evaluate the effect of these factors represented by nasal allergen challenge (spe...

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Autores principales: Panek, Michał Gabriel, Karbownik, Michał Seweryn, Górski, Karol Maksymilian, Koćwin, Marcelina, Kardas, Grzegorz, Marynowski, Mateusz, Kuna, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250282/
https://www.ncbi.nlm.nih.gov/pubmed/35800124
http://dx.doi.org/10.1002/clt2.12172
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author Panek, Michał Gabriel
Karbownik, Michał Seweryn
Górski, Karol Maksymilian
Koćwin, Marcelina
Kardas, Grzegorz
Marynowski, Mateusz
Kuna, Piotr
author_facet Panek, Michał Gabriel
Karbownik, Michał Seweryn
Górski, Karol Maksymilian
Koćwin, Marcelina
Kardas, Grzegorz
Marynowski, Mateusz
Kuna, Piotr
author_sort Panek, Michał Gabriel
collection PubMed
description BACKGROUND: Asthma is a heterogeneous chronic inflammatory disease of the bronchi, the course of which is significantly influenced by extrinsic factors (specific and non‐specific). METHODS: The aim of this study was to evaluate the effect of these factors represented by nasal allergen challenge (specific factors) and methacholine challenge test (non‐specific) on changes in mRNA expression of genes encoding the TGF‐β (TGF‐β1 and TGF‐β3)‒Smad (mitogen‐activated protein kinase 1/3 [MPK1/3], Smad1/3/6/7) signaling pathway in asthmatic patients. RESULTS: Seventy‐five subjects were included in the study, of whom 27 were applied an intranasal allergen provocation and 48 a methacholine provocation. There were 9 men and 18 women in the intranasal provocation group, and 17 men and 31 women in the methacholine test group. We found that both examined the types of challenges contributed to changes in the relative expression of genes of the TGF‐β (TGF‐β1 and TGF‐β3)‒Smad (MPK1/3, Smad1/3/6/7) signaling pathway in asthmatic patients. A decrease was noted for MAPK1, MAPK3, Smad3, Smad6, and Smad7 genes and an increase of up to 2.5 times for TGF‐β1 gene. CONCLUSIONS: Our experiment allows us to conclude that the change in the mRNA expression of the TGF‐β1–MPK1/3 and Smad3/6/7 genes occurs after an intranasal allergen and bronchial methacholine challenge.
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spelling pubmed-92502822022-07-06 New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma Panek, Michał Gabriel Karbownik, Michał Seweryn Górski, Karol Maksymilian Koćwin, Marcelina Kardas, Grzegorz Marynowski, Mateusz Kuna, Piotr Clin Transl Allergy Original Article BACKGROUND: Asthma is a heterogeneous chronic inflammatory disease of the bronchi, the course of which is significantly influenced by extrinsic factors (specific and non‐specific). METHODS: The aim of this study was to evaluate the effect of these factors represented by nasal allergen challenge (specific factors) and methacholine challenge test (non‐specific) on changes in mRNA expression of genes encoding the TGF‐β (TGF‐β1 and TGF‐β3)‒Smad (mitogen‐activated protein kinase 1/3 [MPK1/3], Smad1/3/6/7) signaling pathway in asthmatic patients. RESULTS: Seventy‐five subjects were included in the study, of whom 27 were applied an intranasal allergen provocation and 48 a methacholine provocation. There were 9 men and 18 women in the intranasal provocation group, and 17 men and 31 women in the methacholine test group. We found that both examined the types of challenges contributed to changes in the relative expression of genes of the TGF‐β (TGF‐β1 and TGF‐β3)‒Smad (MPK1/3, Smad1/3/6/7) signaling pathway in asthmatic patients. A decrease was noted for MAPK1, MAPK3, Smad3, Smad6, and Smad7 genes and an increase of up to 2.5 times for TGF‐β1 gene. CONCLUSIONS: Our experiment allows us to conclude that the change in the mRNA expression of the TGF‐β1–MPK1/3 and Smad3/6/7 genes occurs after an intranasal allergen and bronchial methacholine challenge. John Wiley and Sons Inc. 2022-07-02 /pmc/articles/PMC9250282/ /pubmed/35800124 http://dx.doi.org/10.1002/clt2.12172 Text en © 2022 The Authors. Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Panek, Michał Gabriel
Karbownik, Michał Seweryn
Górski, Karol Maksymilian
Koćwin, Marcelina
Kardas, Grzegorz
Marynowski, Mateusz
Kuna, Piotr
New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma
title New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma
title_full New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma
title_fullStr New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma
title_full_unstemmed New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma
title_short New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma
title_sort new insights into the regulation of tgf‐β/smad and mpk signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250282/
https://www.ncbi.nlm.nih.gov/pubmed/35800124
http://dx.doi.org/10.1002/clt2.12172
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