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CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis
Rheumatoid arthritis (RA) is a chronic systematicness autoimmunity disease with joint inflammation. RA etiology is still unknown. Early and exact diagnosing is still hard to reach. In the paper, we purposed to discover novel diagnosis biological marker for RA. Two open, usable gene expression profil...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250429/ https://www.ncbi.nlm.nih.gov/pubmed/35789606 http://dx.doi.org/10.1155/2022/2431976 |
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author | Wang, Zhi-wen Zhang, Li-jun Zhuang, Yu Lv, Zhi-fen Tan, Zhi-ming |
author_facet | Wang, Zhi-wen Zhang, Li-jun Zhuang, Yu Lv, Zhi-fen Tan, Zhi-ming |
author_sort | Wang, Zhi-wen |
collection | PubMed |
description | Rheumatoid arthritis (RA) is a chronic systematicness autoimmunity disease with joint inflammation. RA etiology is still unknown. Early and exact diagnosing is still hard to reach. In the paper, we purposed to discover novel diagnosis biological marker for RA. Two open, usable gene expression profiles of human RA as well as controlled specimens (dataset GSE17755 as well as GSE93272) were downloaded from the GEO database. Differentially expressed genes (DEGs) were screened between 331 RA and 88 control samples. Functional enrichment analysis was applied to explore the possible function of DEGs. Expression levels as well as diagnosis values of biological marker in RA were further verified in our cohort by the use of RT-PCR and ROC assays. We identified 13 DEGs between RA samples and control samples. 13 DEGs were remarkably abundant in NF-kappa B signal pathway. Among the 13 DEGs, CKS2, S100A12, LY96, and ANXA3 exhibited a strong diagnostic ability in screening RA specimens from normal specimens using all AUC > 0.8. Moreover, we confirmed that the expression of CKS2 and S100A12 was distinctly upregulated in RA specimens contrasted to normal specimens. Overall, serum CKS2 and S100A12 could be used as novel diagnosis biological markers for RA patients. |
format | Online Article Text |
id | pubmed-9250429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-92504292022-07-03 CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis Wang, Zhi-wen Zhang, Li-jun Zhuang, Yu Lv, Zhi-fen Tan, Zhi-ming Dis Markers Research Article Rheumatoid arthritis (RA) is a chronic systematicness autoimmunity disease with joint inflammation. RA etiology is still unknown. Early and exact diagnosing is still hard to reach. In the paper, we purposed to discover novel diagnosis biological marker for RA. Two open, usable gene expression profiles of human RA as well as controlled specimens (dataset GSE17755 as well as GSE93272) were downloaded from the GEO database. Differentially expressed genes (DEGs) were screened between 331 RA and 88 control samples. Functional enrichment analysis was applied to explore the possible function of DEGs. Expression levels as well as diagnosis values of biological marker in RA were further verified in our cohort by the use of RT-PCR and ROC assays. We identified 13 DEGs between RA samples and control samples. 13 DEGs were remarkably abundant in NF-kappa B signal pathway. Among the 13 DEGs, CKS2, S100A12, LY96, and ANXA3 exhibited a strong diagnostic ability in screening RA specimens from normal specimens using all AUC > 0.8. Moreover, we confirmed that the expression of CKS2 and S100A12 was distinctly upregulated in RA specimens contrasted to normal specimens. Overall, serum CKS2 and S100A12 could be used as novel diagnosis biological markers for RA patients. Hindawi 2022-06-25 /pmc/articles/PMC9250429/ /pubmed/35789606 http://dx.doi.org/10.1155/2022/2431976 Text en Copyright © 2022 Zhi-wen Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Zhi-wen Zhang, Li-jun Zhuang, Yu Lv, Zhi-fen Tan, Zhi-ming CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis |
title | CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis |
title_full | CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis |
title_fullStr | CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis |
title_full_unstemmed | CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis |
title_short | CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis |
title_sort | cks2 and s100a12: two novel diagnostic biomarkers for rheumatoid arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250429/ https://www.ncbi.nlm.nih.gov/pubmed/35789606 http://dx.doi.org/10.1155/2022/2431976 |
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