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Predicting high recombinant protein producer strains of Pichia pastoris Mut(S) using the oxygen transfer rate as an indicator of metabolic burden

The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is a widely used host for recombinant protein production. In this study, a clonal library of P. pastoris Mut(S) strains ((S) indicates slow methanol utilization) was screened for high green fluorescent protein (GFP) production. The expr...

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Detalles Bibliográficos
Autores principales:	Wollborn, David, Munkler, Lara Pauline, Horstmann, Rebekka, Germer, Andrea, Blank, Lars Mathias, Büchs, Jochen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250517/ https://www.ncbi.nlm.nih.gov/pubmed/35780248 http://dx.doi.org/10.1038/s41598-022-15086-w

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