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Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges

Bacteria are part of the insect gut system and influence many physiological traits of their host. Gut bacteria may even reduce or block the transmission of arboviruses in several species of arthropod vectors. Culicoides biting midges are important arboviral vectors of several livestock and wildlife...

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Autores principales: Möhlmann, Tim W. R., ter Braak, Cajo J. F., te Beest, Dennis E., Hendriks, Marc, Nijhuis, Els H., Warris, Sven, Drolet, Barbara S., van Overbeek, Leo, Koenraadt, Constantianus J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250918/
https://www.ncbi.nlm.nih.gov/pubmed/34436640
http://dx.doi.org/10.1007/s00248-021-01822-8
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author Möhlmann, Tim W. R.
ter Braak, Cajo J. F.
te Beest, Dennis E.
Hendriks, Marc
Nijhuis, Els H.
Warris, Sven
Drolet, Barbara S.
van Overbeek, Leo
Koenraadt, Constantianus J. M.
author_facet Möhlmann, Tim W. R.
ter Braak, Cajo J. F.
te Beest, Dennis E.
Hendriks, Marc
Nijhuis, Els H.
Warris, Sven
Drolet, Barbara S.
van Overbeek, Leo
Koenraadt, Constantianus J. M.
author_sort Möhlmann, Tim W. R.
collection PubMed
description Bacteria are part of the insect gut system and influence many physiological traits of their host. Gut bacteria may even reduce or block the transmission of arboviruses in several species of arthropod vectors. Culicoides biting midges are important arboviral vectors of several livestock and wildlife diseases, yet limited information is available on their gut bacterial communities. Addressing this gap will help inform how these communities can be manipulated and ultimately used as novel tools to control pathogens. To assess how bacterial communities change during the life stages of lab-reared C. nubeculosus and C. sonorensis, endosymbiotic bacteria were identified using Illumina sequencing of 16S rRNA and taxonomically characterised. Analyses were conducted to determine how gut bacterial communities in adults are influenced by species identity and geographic distance among biting midge populations. Communities of the two lab-reared Culicoides species significantly changed after pupation and with maturation into 6-day-old adults. Pseudomonas, Burkholderiaceae and Leucobacter bacteria were part of a core community that was trans-stadially transmitted and found throughout their life cycle. Among field-collected biting midges, the bacterial communities were unique for almost each species. Cardinium, Rickettsia and Wolbachia were some of the most abundant bacteria in midges collected from wetlands. Only Pseudomonas was present in high relative abundance in all field-collected species. In this study, species identity, as well as geographic distance, influenced the gut bacterial communities and may partly explain known inter- and intra-species variability in vector competence. Additionally, stably associated bacterial species could be candidates for paratransgenic strategies to control vector-borne pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00248-021-01822-8.
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spelling pubmed-92509182022-07-05 Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges Möhlmann, Tim W. R. ter Braak, Cajo J. F. te Beest, Dennis E. Hendriks, Marc Nijhuis, Els H. Warris, Sven Drolet, Barbara S. van Overbeek, Leo Koenraadt, Constantianus J. M. Microb Ecol Host Microbe Interactions Bacteria are part of the insect gut system and influence many physiological traits of their host. Gut bacteria may even reduce or block the transmission of arboviruses in several species of arthropod vectors. Culicoides biting midges are important arboviral vectors of several livestock and wildlife diseases, yet limited information is available on their gut bacterial communities. Addressing this gap will help inform how these communities can be manipulated and ultimately used as novel tools to control pathogens. To assess how bacterial communities change during the life stages of lab-reared C. nubeculosus and C. sonorensis, endosymbiotic bacteria were identified using Illumina sequencing of 16S rRNA and taxonomically characterised. Analyses were conducted to determine how gut bacterial communities in adults are influenced by species identity and geographic distance among biting midge populations. Communities of the two lab-reared Culicoides species significantly changed after pupation and with maturation into 6-day-old adults. Pseudomonas, Burkholderiaceae and Leucobacter bacteria were part of a core community that was trans-stadially transmitted and found throughout their life cycle. Among field-collected biting midges, the bacterial communities were unique for almost each species. Cardinium, Rickettsia and Wolbachia were some of the most abundant bacteria in midges collected from wetlands. Only Pseudomonas was present in high relative abundance in all field-collected species. In this study, species identity, as well as geographic distance, influenced the gut bacterial communities and may partly explain known inter- and intra-species variability in vector competence. Additionally, stably associated bacterial species could be candidates for paratransgenic strategies to control vector-borne pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00248-021-01822-8. Springer US 2021-08-26 2022 /pmc/articles/PMC9250918/ /pubmed/34436640 http://dx.doi.org/10.1007/s00248-021-01822-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Host Microbe Interactions
Möhlmann, Tim W. R.
ter Braak, Cajo J. F.
te Beest, Dennis E.
Hendriks, Marc
Nijhuis, Els H.
Warris, Sven
Drolet, Barbara S.
van Overbeek, Leo
Koenraadt, Constantianus J. M.
Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges
title Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges
title_full Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges
title_fullStr Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges
title_full_unstemmed Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges
title_short Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges
title_sort species identity, life history, and geographic distance influence gut bacterial communities in lab-reared and european field-collected culicoides biting midges
topic Host Microbe Interactions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250918/
https://www.ncbi.nlm.nih.gov/pubmed/34436640
http://dx.doi.org/10.1007/s00248-021-01822-8
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