Cargando…

Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar

BACKGROUND: Constipation is a common functional gastrointestinal disorder. Medicines derived from nature are routinely used to treat it. The present study evaluates the gut stimulatory activity of Aloe musabbar (processed powder of Aloe vera) using in vitro and in vivo models for gut stimulatory act...

Descripción completa

Detalles Bibliográficos
Autores principales: Tandur, Vijayamahantesh K., Inamdar, Mohammed Naseeruddin, Orfali, Raha, Asdaq, Syed Mohammed Basheeruddin, Rabbani, Syed Imam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251092/
https://www.ncbi.nlm.nih.gov/pubmed/35795288
http://dx.doi.org/10.1155/2022/4163008
_version_ 1784739961993428992
author Tandur, Vijayamahantesh K.
Inamdar, Mohammed Naseeruddin
Orfali, Raha
Asdaq, Syed Mohammed Basheeruddin
Rabbani, Syed Imam
author_facet Tandur, Vijayamahantesh K.
Inamdar, Mohammed Naseeruddin
Orfali, Raha
Asdaq, Syed Mohammed Basheeruddin
Rabbani, Syed Imam
author_sort Tandur, Vijayamahantesh K.
collection PubMed
description BACKGROUND: Constipation is a common functional gastrointestinal disorder. Medicines derived from nature are routinely used to treat it. The present study evaluates the gut stimulatory activity of Aloe musabbar (processed powder of Aloe vera) using in vitro and in vivo models for gut stimulatory activity. MATERIALS AND METHODS: In vitro tests were conducted on isolated rat colon, guinea pig ileum, and rabbit jejunum, while in vivo study was performed using mice intestinal transit time. Aloe musabbar (A. musabbar) was tested at doses 0.2–200 mg/mL (in-vitro study) and 86.6 mg/kg (in vivo study). In vitro studies were done in the presence and absence of atropine sulphate (1 ng/ml). The results were statistically analyzed, and p < 0.05 was considered to indicate the significance. RESULTS: A. musabbar exhibited dose-dependent increase in the smooth muscle contraction of isolated gut tissues. Presence of atropine minimized the contractile responses and shifted the dose-response curves towards the right-hand side. The intestinal transit time in mice was observed to be increased significantly (p < 0.01) in A. musabbar-treated animals, when compared with normal animals. CONCLUSION: A mild smooth muscle contraction induced by A. musabbar suggests that it can stimulate intestinal bowel movement without causing spasms. The diminished responses in the presence of atropine indicated that the gut stimulatory activity could be mediated partially through parasympathetic innervations. More studies are needed to determine the precise mechanism of action including the specific active ingredient responsible for the gut stimulatory activity.
format Online
Article
Text
id pubmed-9251092
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-92510922022-07-05 Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar Tandur, Vijayamahantesh K. Inamdar, Mohammed Naseeruddin Orfali, Raha Asdaq, Syed Mohammed Basheeruddin Rabbani, Syed Imam Evid Based Complement Alternat Med Research Article BACKGROUND: Constipation is a common functional gastrointestinal disorder. Medicines derived from nature are routinely used to treat it. The present study evaluates the gut stimulatory activity of Aloe musabbar (processed powder of Aloe vera) using in vitro and in vivo models for gut stimulatory activity. MATERIALS AND METHODS: In vitro tests were conducted on isolated rat colon, guinea pig ileum, and rabbit jejunum, while in vivo study was performed using mice intestinal transit time. Aloe musabbar (A. musabbar) was tested at doses 0.2–200 mg/mL (in-vitro study) and 86.6 mg/kg (in vivo study). In vitro studies were done in the presence and absence of atropine sulphate (1 ng/ml). The results were statistically analyzed, and p < 0.05 was considered to indicate the significance. RESULTS: A. musabbar exhibited dose-dependent increase in the smooth muscle contraction of isolated gut tissues. Presence of atropine minimized the contractile responses and shifted the dose-response curves towards the right-hand side. The intestinal transit time in mice was observed to be increased significantly (p < 0.01) in A. musabbar-treated animals, when compared with normal animals. CONCLUSION: A mild smooth muscle contraction induced by A. musabbar suggests that it can stimulate intestinal bowel movement without causing spasms. The diminished responses in the presence of atropine indicated that the gut stimulatory activity could be mediated partially through parasympathetic innervations. More studies are needed to determine the precise mechanism of action including the specific active ingredient responsible for the gut stimulatory activity. Hindawi 2022-06-26 /pmc/articles/PMC9251092/ /pubmed/35795288 http://dx.doi.org/10.1155/2022/4163008 Text en Copyright © 2022 Vijayamahantesh K. Tandur et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tandur, Vijayamahantesh K.
Inamdar, Mohammed Naseeruddin
Orfali, Raha
Asdaq, Syed Mohammed Basheeruddin
Rabbani, Syed Imam
Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar
title Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar
title_full Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar
title_fullStr Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar
title_full_unstemmed Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar
title_short Preclinical Studies to Evaluate the Gut Stimulatory Activity of Aloe Musabbar
title_sort preclinical studies to evaluate the gut stimulatory activity of aloe musabbar
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251092/
https://www.ncbi.nlm.nih.gov/pubmed/35795288
http://dx.doi.org/10.1155/2022/4163008
work_keys_str_mv AT tandurvijayamahanteshk preclinicalstudiestoevaluatethegutstimulatoryactivityofaloemusabbar
AT inamdarmohammednaseeruddin preclinicalstudiestoevaluatethegutstimulatoryactivityofaloemusabbar
AT orfaliraha preclinicalstudiestoevaluatethegutstimulatoryactivityofaloemusabbar
AT asdaqsyedmohammedbasheeruddin preclinicalstudiestoevaluatethegutstimulatoryactivityofaloemusabbar
AT rabbanisyedimam preclinicalstudiestoevaluatethegutstimulatoryactivityofaloemusabbar