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Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation
Objective: Andrographis paniculata (Burm.f.) Nees is a medicinal plant that has been traditionally used as an anti-inflammatory and antibacterial remedy for several conditions. Andrographolide (AG), the active constituent of A. paniculata (Burm.f.) Nees, has anti-lipidic and anti-inflammatory proper...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251584/ https://www.ncbi.nlm.nih.gov/pubmed/35543243 http://dx.doi.org/10.1042/BSR20212812 |
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author | Shi, Shuai Ji, Xinyu Shi, Jingjing Shi, Shuqing She, Fei Zhang, Qiuyan Dong, Yu Cui, Hanming Hu, Yuanhui |
author_facet | Shi, Shuai Ji, Xinyu Shi, Jingjing Shi, Shuqing She, Fei Zhang, Qiuyan Dong, Yu Cui, Hanming Hu, Yuanhui |
author_sort | Shi, Shuai |
collection | PubMed |
description | Objective: Andrographis paniculata (Burm.f.) Nees is a medicinal plant that has been traditionally used as an anti-inflammatory and antibacterial remedy for several conditions. Andrographolide (AG), the active constituent of A. paniculata (Burm.f.) Nees, has anti-lipidic and anti-inflammatory properties as well as cardiovascular protective effects. The present study aimed to explore the effects of AG on the progression of atherosclerosis and to investigate related mechanisms via network pharmacology. Materials and methods: Compound-related information was obtained from the PubChem database. Potential target genes were identified using STITCH, SwissTargetPrediction, Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine, and Comparative Toxicogenomics Database. Genes involved in atherosclerosis were obtained from DisGeNet and compared with AG target genes to obtain an overlapping set. Protein–protein interactions were determined by STRING. Gene ontology (GO) analysis was performed at WebGestalt, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment was analyzed using Metascape. The final network showing the relationship between compounds, targets, and pathways was constructed using Cytoscape. After that, oxLDL-induced RAW264.7 cells were used to further validate a part of the network pharmacology results. Result: Eighty-one potential AG target genes were identified. PPI, GO, and KEGG enrichment revealed genes closely related to tumor progression, lipid transport, inflammation, and related pathways. AG improves the reverse cholesterol transport (RCT) through NF-κB/CEBPB/PPARG signaling in oxLDL-induced RAW264.7 cells. Conclusion: We successfully predict AG’s potential targets and pathways in atherosclerosis and illustrate the mechanism of action. AG may regulate NF-κB/CEBPB/PPARG signaling to alleviate atherosclerosis. |
format | Online Article Text |
id | pubmed-9251584 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92515842022-07-14 Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation Shi, Shuai Ji, Xinyu Shi, Jingjing Shi, Shuqing She, Fei Zhang, Qiuyan Dong, Yu Cui, Hanming Hu, Yuanhui Biosci Rep Cardiovascular System & Vascular Biology Objective: Andrographis paniculata (Burm.f.) Nees is a medicinal plant that has been traditionally used as an anti-inflammatory and antibacterial remedy for several conditions. Andrographolide (AG), the active constituent of A. paniculata (Burm.f.) Nees, has anti-lipidic and anti-inflammatory properties as well as cardiovascular protective effects. The present study aimed to explore the effects of AG on the progression of atherosclerosis and to investigate related mechanisms via network pharmacology. Materials and methods: Compound-related information was obtained from the PubChem database. Potential target genes were identified using STITCH, SwissTargetPrediction, Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine, and Comparative Toxicogenomics Database. Genes involved in atherosclerosis were obtained from DisGeNet and compared with AG target genes to obtain an overlapping set. Protein–protein interactions were determined by STRING. Gene ontology (GO) analysis was performed at WebGestalt, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment was analyzed using Metascape. The final network showing the relationship between compounds, targets, and pathways was constructed using Cytoscape. After that, oxLDL-induced RAW264.7 cells were used to further validate a part of the network pharmacology results. Result: Eighty-one potential AG target genes were identified. PPI, GO, and KEGG enrichment revealed genes closely related to tumor progression, lipid transport, inflammation, and related pathways. AG improves the reverse cholesterol transport (RCT) through NF-κB/CEBPB/PPARG signaling in oxLDL-induced RAW264.7 cells. Conclusion: We successfully predict AG’s potential targets and pathways in atherosclerosis and illustrate the mechanism of action. AG may regulate NF-κB/CEBPB/PPARG signaling to alleviate atherosclerosis. Portland Press Ltd. 2022-07-01 /pmc/articles/PMC9251584/ /pubmed/35543243 http://dx.doi.org/10.1042/BSR20212812 Text en © 2022 The Author(s). https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Cardiovascular System & Vascular Biology Shi, Shuai Ji, Xinyu Shi, Jingjing Shi, Shuqing She, Fei Zhang, Qiuyan Dong, Yu Cui, Hanming Hu, Yuanhui Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation |
title | Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation |
title_full | Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation |
title_fullStr | Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation |
title_full_unstemmed | Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation |
title_short | Andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation |
title_sort | andrographolide in atherosclerosis: integrating network pharmacology and in vitro pharmacological evaluation |
topic | Cardiovascular System & Vascular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251584/ https://www.ncbi.nlm.nih.gov/pubmed/35543243 http://dx.doi.org/10.1042/BSR20212812 |
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