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A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component

Beyond its role in cellular homeostasis, autophagy plays anti‐ and promicrobial roles in host–microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, mic...

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Autores principales: Leong, Jia Xuan, Raffeiner, Margot, Spinti, Daniela, Langin, Gautier, Franz‐Wachtel, Mirita, Guzman, Andrew R, Kim, Jung‐Gun, Pandey, Pooja, Minina, Alyona E, Macek, Boris, Hafrén, Anders, Bozkurt, Tolga O, Mudgett, Mary Beth, Börnke, Frederik, Hofius, Daniel, Üstün, Suayib
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251887/
https://www.ncbi.nlm.nih.gov/pubmed/35620914
http://dx.doi.org/10.15252/embj.2021110352
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author Leong, Jia Xuan
Raffeiner, Margot
Spinti, Daniela
Langin, Gautier
Franz‐Wachtel, Mirita
Guzman, Andrew R
Kim, Jung‐Gun
Pandey, Pooja
Minina, Alyona E
Macek, Boris
Hafrén, Anders
Bozkurt, Tolga O
Mudgett, Mary Beth
Börnke, Frederik
Hofius, Daniel
Üstün, Suayib
author_facet Leong, Jia Xuan
Raffeiner, Margot
Spinti, Daniela
Langin, Gautier
Franz‐Wachtel, Mirita
Guzman, Andrew R
Kim, Jung‐Gun
Pandey, Pooja
Minina, Alyona E
Macek, Boris
Hafrén, Anders
Bozkurt, Tolga O
Mudgett, Mary Beth
Börnke, Frederik
Hofius, Daniel
Üstün, Suayib
author_sort Leong, Jia Xuan
collection PubMed
description Beyond its role in cellular homeostasis, autophagy plays anti‐ and promicrobial roles in host–microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well‐described in animals, the extent to which xenophagy contributes to plant–bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type‐III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense‐related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense‐related autophagy in plant–bacteria interactions.
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spelling pubmed-92518872022-07-11 A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component Leong, Jia Xuan Raffeiner, Margot Spinti, Daniela Langin, Gautier Franz‐Wachtel, Mirita Guzman, Andrew R Kim, Jung‐Gun Pandey, Pooja Minina, Alyona E Macek, Boris Hafrén, Anders Bozkurt, Tolga O Mudgett, Mary Beth Börnke, Frederik Hofius, Daniel Üstün, Suayib EMBO J Articles Beyond its role in cellular homeostasis, autophagy plays anti‐ and promicrobial roles in host–microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well‐described in animals, the extent to which xenophagy contributes to plant–bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type‐III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense‐related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense‐related autophagy in plant–bacteria interactions. John Wiley and Sons Inc. 2022-05-27 /pmc/articles/PMC9251887/ /pubmed/35620914 http://dx.doi.org/10.15252/embj.2021110352 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Leong, Jia Xuan
Raffeiner, Margot
Spinti, Daniela
Langin, Gautier
Franz‐Wachtel, Mirita
Guzman, Andrew R
Kim, Jung‐Gun
Pandey, Pooja
Minina, Alyona E
Macek, Boris
Hafrén, Anders
Bozkurt, Tolga O
Mudgett, Mary Beth
Börnke, Frederik
Hofius, Daniel
Üstün, Suayib
A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
title A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
title_full A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
title_fullStr A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
title_full_unstemmed A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
title_short A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
title_sort bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251887/
https://www.ncbi.nlm.nih.gov/pubmed/35620914
http://dx.doi.org/10.15252/embj.2021110352
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