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A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component
Beyond its role in cellular homeostasis, autophagy plays anti‐ and promicrobial roles in host–microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, mic...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251887/ https://www.ncbi.nlm.nih.gov/pubmed/35620914 http://dx.doi.org/10.15252/embj.2021110352 |
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author | Leong, Jia Xuan Raffeiner, Margot Spinti, Daniela Langin, Gautier Franz‐Wachtel, Mirita Guzman, Andrew R Kim, Jung‐Gun Pandey, Pooja Minina, Alyona E Macek, Boris Hafrén, Anders Bozkurt, Tolga O Mudgett, Mary Beth Börnke, Frederik Hofius, Daniel Üstün, Suayib |
author_facet | Leong, Jia Xuan Raffeiner, Margot Spinti, Daniela Langin, Gautier Franz‐Wachtel, Mirita Guzman, Andrew R Kim, Jung‐Gun Pandey, Pooja Minina, Alyona E Macek, Boris Hafrén, Anders Bozkurt, Tolga O Mudgett, Mary Beth Börnke, Frederik Hofius, Daniel Üstün, Suayib |
author_sort | Leong, Jia Xuan |
collection | PubMed |
description | Beyond its role in cellular homeostasis, autophagy plays anti‐ and promicrobial roles in host–microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well‐described in animals, the extent to which xenophagy contributes to plant–bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type‐III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense‐related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense‐related autophagy in plant–bacteria interactions. |
format | Online Article Text |
id | pubmed-9251887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92518872022-07-11 A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component Leong, Jia Xuan Raffeiner, Margot Spinti, Daniela Langin, Gautier Franz‐Wachtel, Mirita Guzman, Andrew R Kim, Jung‐Gun Pandey, Pooja Minina, Alyona E Macek, Boris Hafrén, Anders Bozkurt, Tolga O Mudgett, Mary Beth Börnke, Frederik Hofius, Daniel Üstün, Suayib EMBO J Articles Beyond its role in cellular homeostasis, autophagy plays anti‐ and promicrobial roles in host–microbe interactions, both in animals and plants. One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy. Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination. Although well‐described in animals, the extent to which xenophagy contributes to plant–bacteria interactions remains unknown. Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type‐III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection. Intriguingly, XopL is targeted for degradation by defense‐related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery. Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense‐related autophagy in plant–bacteria interactions. John Wiley and Sons Inc. 2022-05-27 /pmc/articles/PMC9251887/ /pubmed/35620914 http://dx.doi.org/10.15252/embj.2021110352 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Leong, Jia Xuan Raffeiner, Margot Spinti, Daniela Langin, Gautier Franz‐Wachtel, Mirita Guzman, Andrew R Kim, Jung‐Gun Pandey, Pooja Minina, Alyona E Macek, Boris Hafrén, Anders Bozkurt, Tolga O Mudgett, Mary Beth Börnke, Frederik Hofius, Daniel Üstün, Suayib A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component |
title | A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component |
title_full | A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component |
title_fullStr | A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component |
title_full_unstemmed | A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component |
title_short | A bacterial effector counteracts host autophagy by promoting degradation of an autophagy component |
title_sort | bacterial effector counteracts host autophagy by promoting degradation of an autophagy component |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251887/ https://www.ncbi.nlm.nih.gov/pubmed/35620914 http://dx.doi.org/10.15252/embj.2021110352 |
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