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A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake
Necrotic enteritis (NE) in poultry is an opportunistic infection caused by Clostridium perfringens. Well-known as a multifactorial disease, NE development is under the influence of a wide range of environmental risk factors that promote the proliferation of pathogenic C. perfringens at the expense o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251903/ https://www.ncbi.nlm.nih.gov/pubmed/35795645 http://dx.doi.org/10.3389/fphys.2022.788592 |
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author | He, Wanwei Goes, Emanuele C. Wakaruk, Jeremy Barreda, Daniel R. Korver, Douglas R. |
author_facet | He, Wanwei Goes, Emanuele C. Wakaruk, Jeremy Barreda, Daniel R. Korver, Douglas R. |
author_sort | He, Wanwei |
collection | PubMed |
description | Necrotic enteritis (NE) in poultry is an opportunistic infection caused by Clostridium perfringens. Well-known as a multifactorial disease, NE development is under the influence of a wide range of environmental risk factors that promote the proliferation of pathogenic C. perfringens at the expense of nonpathogenic strains. Current in vivo NE challenge models typically incorporate pre-exposure to disease risk factors, in combination with exogenous C. perfringens inoculation. Our goal was to enhance current models using a natural uptake of C. perfringens from the barn environment to produce a subclinical infection. We incorporated access to litter, coccidial exposure (either 10× or 15× of the manufacturer-recommended Coccivac B52 Eimeria vaccine challenge; provided unspecified doses of E. acervulina, E. mivati, E. tenella, and two strains of E. maxima), feed composition, and feed withdrawal stress, and achieved the commonly observed NE infection peak at 3 weeks post-hatch. NE severity was evaluated based on gut lesion pathology, clinical signs, and mortality rate. Under cage-reared conditions, 15× coccidial vaccine-challenged birds showed overall NE lesion prevalence that was 8-fold higher than 10× coccidial vaccine-challenged birds. NE-associated mortality was observed only in a floor-reared flock after a 15× coccidial vaccine challenge. |
format | Online Article Text |
id | pubmed-9251903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92519032022-07-05 A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake He, Wanwei Goes, Emanuele C. Wakaruk, Jeremy Barreda, Daniel R. Korver, Douglas R. Front Physiol Physiology Necrotic enteritis (NE) in poultry is an opportunistic infection caused by Clostridium perfringens. Well-known as a multifactorial disease, NE development is under the influence of a wide range of environmental risk factors that promote the proliferation of pathogenic C. perfringens at the expense of nonpathogenic strains. Current in vivo NE challenge models typically incorporate pre-exposure to disease risk factors, in combination with exogenous C. perfringens inoculation. Our goal was to enhance current models using a natural uptake of C. perfringens from the barn environment to produce a subclinical infection. We incorporated access to litter, coccidial exposure (either 10× or 15× of the manufacturer-recommended Coccivac B52 Eimeria vaccine challenge; provided unspecified doses of E. acervulina, E. mivati, E. tenella, and two strains of E. maxima), feed composition, and feed withdrawal stress, and achieved the commonly observed NE infection peak at 3 weeks post-hatch. NE severity was evaluated based on gut lesion pathology, clinical signs, and mortality rate. Under cage-reared conditions, 15× coccidial vaccine-challenged birds showed overall NE lesion prevalence that was 8-fold higher than 10× coccidial vaccine-challenged birds. NE-associated mortality was observed only in a floor-reared flock after a 15× coccidial vaccine challenge. Frontiers Media S.A. 2022-06-08 /pmc/articles/PMC9251903/ /pubmed/35795645 http://dx.doi.org/10.3389/fphys.2022.788592 Text en Copyright © 2022 He, Goes, Wakaruk, Barreda and Korver. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology He, Wanwei Goes, Emanuele C. Wakaruk, Jeremy Barreda, Daniel R. Korver, Douglas R. A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake |
title | A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake |
title_full | A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake |
title_fullStr | A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake |
title_full_unstemmed | A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake |
title_short | A Poultry Subclinical Necrotic Enteritis Disease Model Based on Natural Clostridium perfringens Uptake |
title_sort | poultry subclinical necrotic enteritis disease model based on natural clostridium perfringens uptake |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251903/ https://www.ncbi.nlm.nih.gov/pubmed/35795645 http://dx.doi.org/10.3389/fphys.2022.788592 |
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