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Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells

Hematopoiesis takes place in the bone marrow and is supported by a complex cellular and molecular network in the bone marrow microenvironment. Commonly used models of the human bone marrow microenvironment include murine models and two-dimensional and three-dimensional tissue cultures. While these m...

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Autores principales: Sharipol, Azmeer, Lesch, Maggie L., Soto, Celia A., Frisch, Benjamin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252162/
https://www.ncbi.nlm.nih.gov/pubmed/35795163
http://dx.doi.org/10.3389/fbioe.2022.855777
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author Sharipol, Azmeer
Lesch, Maggie L.
Soto, Celia A.
Frisch, Benjamin J.
author_facet Sharipol, Azmeer
Lesch, Maggie L.
Soto, Celia A.
Frisch, Benjamin J.
author_sort Sharipol, Azmeer
collection PubMed
description Hematopoiesis takes place in the bone marrow and is supported by a complex cellular and molecular network in the bone marrow microenvironment. Commonly used models of the human bone marrow microenvironment include murine models and two-dimensional and three-dimensional tissue cultures. While these model systems have led to critical advances in the field, they fail to recapitulate many aspects of the human bone marrow. This has limited our understanding of human bone marrow pathophysiology and has led to deficiencies in therapy for many bone marrow pathologies such as bone marrow failure syndromes and leukemias. Therefore, we have developed a modular murine bone marrow microenvironment-on-chip using a commercially available microfluidic platform. This model includes a vascular channel separated from the bone marrow channel by a semi-porous membrane and incorporates critical components of the bone marrow microenvironment, including osteoblasts, endothelial cells, mesenchymal stem cells, and hematopoietic stem and progenitor cells. This system is capable of maintaining functional hematopoietic stem cells in vitro for at least 14 days at frequencies similar to what is found in the primary bone marrow. The modular nature of this system and its accessibility will allow for acceleration of our understanding of the bone marrow.
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spelling pubmed-92521622022-07-05 Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells Sharipol, Azmeer Lesch, Maggie L. Soto, Celia A. Frisch, Benjamin J. Front Bioeng Biotechnol Bioengineering and Biotechnology Hematopoiesis takes place in the bone marrow and is supported by a complex cellular and molecular network in the bone marrow microenvironment. Commonly used models of the human bone marrow microenvironment include murine models and two-dimensional and three-dimensional tissue cultures. While these model systems have led to critical advances in the field, they fail to recapitulate many aspects of the human bone marrow. This has limited our understanding of human bone marrow pathophysiology and has led to deficiencies in therapy for many bone marrow pathologies such as bone marrow failure syndromes and leukemias. Therefore, we have developed a modular murine bone marrow microenvironment-on-chip using a commercially available microfluidic platform. This model includes a vascular channel separated from the bone marrow channel by a semi-porous membrane and incorporates critical components of the bone marrow microenvironment, including osteoblasts, endothelial cells, mesenchymal stem cells, and hematopoietic stem and progenitor cells. This system is capable of maintaining functional hematopoietic stem cells in vitro for at least 14 days at frequencies similar to what is found in the primary bone marrow. The modular nature of this system and its accessibility will allow for acceleration of our understanding of the bone marrow. Frontiers Media S.A. 2022-06-20 /pmc/articles/PMC9252162/ /pubmed/35795163 http://dx.doi.org/10.3389/fbioe.2022.855777 Text en Copyright © 2022 Sharipol, Lesch, Soto and Frisch. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Sharipol, Azmeer
Lesch, Maggie L.
Soto, Celia A.
Frisch, Benjamin J.
Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells
title Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells
title_full Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells
title_fullStr Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells
title_full_unstemmed Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells
title_short Bone Marrow Microenvironment-On-Chip for Culture of Functional Hematopoietic Stem Cells
title_sort bone marrow microenvironment-on-chip for culture of functional hematopoietic stem cells
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252162/
https://www.ncbi.nlm.nih.gov/pubmed/35795163
http://dx.doi.org/10.3389/fbioe.2022.855777
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