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Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression

BACKGROUND: Troxerutin is a flavonoid compound and possesses potential anti-cancer, antioxidant, and anti-inflammatory activities. Besides, cisplatin is one of the most widely used therapeutic agents, but the clinical uses of cisplatin are often associated with multiple side effects, among which nep...

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Autores principales: Guan, Tongxu, Zheng, Yingce, Jin, Shengzi, Wang, Shuang, Hu, Mengxin, Liu, Xingyao, Huang, Siqi, Liu, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Open Academia 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252313/
https://www.ncbi.nlm.nih.gov/pubmed/35844954
http://dx.doi.org/10.29219/fnr.v66.8469
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author Guan, Tongxu
Zheng, Yingce
Jin, Shengzi
Wang, Shuang
Hu, Mengxin
Liu, Xingyao
Huang, Siqi
Liu, Yun
author_facet Guan, Tongxu
Zheng, Yingce
Jin, Shengzi
Wang, Shuang
Hu, Mengxin
Liu, Xingyao
Huang, Siqi
Liu, Yun
author_sort Guan, Tongxu
collection PubMed
description BACKGROUND: Troxerutin is a flavonoid compound and possesses potential anti-cancer, antioxidant, and anti-inflammatory activities. Besides, cisplatin is one of the most widely used therapeutic agents, but the clinical uses of cisplatin are often associated with multiple side effects, among which nephrotoxicity is more common. OBJECTIVE AND DESIGN: This study explored the protective effects of troxerutin (150 mg kg(−1) day(−1) for 14 days) against cisplatin-induced kidney injury and the potential mechanism using Wistar rats as an experimental mammalian model. RESULTS: We discovered that troxerutin could significantly alleviate cisplatin-induced renal dysfunction, such as increased levels of blood urea nitrogen and creatinine (P < 0.01), as well as improved abnormal renal tissue microstructure and ultrastructure. Additionally, troxerutin significantly decreased malondialdehyde (MDA), hydrogen peroxide (H(2)O(2)), NO, inducible nitric oxide synthase (iNOS) levels (P < 0.01), p-NF-κB p65/NF-κB p65, TNF-α, Pro-IL-1β, IL-6, B cell lymphoma-2 (Bcl-2)/Bcl-xl associated death promoter (Bad), Cytochrome C (Cyt C), Cleaved-caspase 9, Cleaved-caspase 3, and Cleaved-caspase 8 protein levels (P < 0.01) in the kidney tissues of cisplatin-treated rats; and increased superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), total antioxidant capacity (T-AOC) activities (P < 0.01), IL-10, Bcl-2 protein levels (P < 0.01). CONCLUSION: These results suggested that the underlying mechanism might be attributed to the regulation of Phosphoinositide 3 kinase/Protein kinase B (PI3K/AKT) pathway via enhancing MAP4 expression to attenuate cellular apoptosis, alleviating oxidative stress and inflammatory response.
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spelling pubmed-92523132022-07-14 Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression Guan, Tongxu Zheng, Yingce Jin, Shengzi Wang, Shuang Hu, Mengxin Liu, Xingyao Huang, Siqi Liu, Yun Food Nutr Res Original Article BACKGROUND: Troxerutin is a flavonoid compound and possesses potential anti-cancer, antioxidant, and anti-inflammatory activities. Besides, cisplatin is one of the most widely used therapeutic agents, but the clinical uses of cisplatin are often associated with multiple side effects, among which nephrotoxicity is more common. OBJECTIVE AND DESIGN: This study explored the protective effects of troxerutin (150 mg kg(−1) day(−1) for 14 days) against cisplatin-induced kidney injury and the potential mechanism using Wistar rats as an experimental mammalian model. RESULTS: We discovered that troxerutin could significantly alleviate cisplatin-induced renal dysfunction, such as increased levels of blood urea nitrogen and creatinine (P < 0.01), as well as improved abnormal renal tissue microstructure and ultrastructure. Additionally, troxerutin significantly decreased malondialdehyde (MDA), hydrogen peroxide (H(2)O(2)), NO, inducible nitric oxide synthase (iNOS) levels (P < 0.01), p-NF-κB p65/NF-κB p65, TNF-α, Pro-IL-1β, IL-6, B cell lymphoma-2 (Bcl-2)/Bcl-xl associated death promoter (Bad), Cytochrome C (Cyt C), Cleaved-caspase 9, Cleaved-caspase 3, and Cleaved-caspase 8 protein levels (P < 0.01) in the kidney tissues of cisplatin-treated rats; and increased superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), total antioxidant capacity (T-AOC) activities (P < 0.01), IL-10, Bcl-2 protein levels (P < 0.01). CONCLUSION: These results suggested that the underlying mechanism might be attributed to the regulation of Phosphoinositide 3 kinase/Protein kinase B (PI3K/AKT) pathway via enhancing MAP4 expression to attenuate cellular apoptosis, alleviating oxidative stress and inflammatory response. Open Academia 2022-05-24 /pmc/articles/PMC9252313/ /pubmed/35844954 http://dx.doi.org/10.29219/fnr.v66.8469 Text en © 2022 Tongxu Guan et al. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
spellingShingle Original Article
Guan, Tongxu
Zheng, Yingce
Jin, Shengzi
Wang, Shuang
Hu, Mengxin
Liu, Xingyao
Huang, Siqi
Liu, Yun
Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression
title Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression
title_full Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression
title_fullStr Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression
title_full_unstemmed Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression
title_short Troxerutin alleviates kidney injury in rats via PI3K/AKT pathway by enhancing MAP4 expression
title_sort troxerutin alleviates kidney injury in rats via pi3k/akt pathway by enhancing map4 expression
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252313/
https://www.ncbi.nlm.nih.gov/pubmed/35844954
http://dx.doi.org/10.29219/fnr.v66.8469
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