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A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?

The gap between in vitro and in vivo assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed in vivo. A...

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Autores principales: Lampejo, Arinola O., Hu, Nien-Wen, Lucas, Daniela, Lomel, Banks M., Nguyen, Christian M., Dominguez, Carmen C., Ren, Bing, Huang, Yong, Murfee, Walter L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252339/
https://www.ncbi.nlm.nih.gov/pubmed/35795159
http://dx.doi.org/10.3389/fbioe.2022.912073
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author Lampejo, Arinola O.
Hu, Nien-Wen
Lucas, Daniela
Lomel, Banks M.
Nguyen, Christian M.
Dominguez, Carmen C.
Ren, Bing
Huang, Yong
Murfee, Walter L.
author_facet Lampejo, Arinola O.
Hu, Nien-Wen
Lucas, Daniela
Lomel, Banks M.
Nguyen, Christian M.
Dominguez, Carmen C.
Ren, Bing
Huang, Yong
Murfee, Walter L.
author_sort Lampejo, Arinola O.
collection PubMed
description The gap between in vitro and in vivo assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed in vivo. A key challenge in model development, however, is determining how to recreate the multi-cell/system functional complexity of a real network environment that integrates endothelial cells, smooth muscle cells, vascular pericytes, lymphatics, nerves, fluid flow, extracellular matrix, and inflammatory cells. The objective of this mini-review is to overview the recent evolution of popular biomimetic modeling approaches for investigating microvascular dynamics. A specific focus will highlight the engineering design requirements needed to match physiological function and the potential for top-down tissue culture methods that maintain complexity. Overall, examples of physiological validation, basic science discoveries, and therapeutic evaluation studies will emphasize the value of tissue culture models and biomimetic model development approaches that fill the gap between in vitro and in vivo assays and guide how vascular biologists and physiologists might think about the microcirculation.
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spelling pubmed-92523392022-07-05 A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology? Lampejo, Arinola O. Hu, Nien-Wen Lucas, Daniela Lomel, Banks M. Nguyen, Christian M. Dominguez, Carmen C. Ren, Bing Huang, Yong Murfee, Walter L. Front Bioeng Biotechnol Bioengineering and Biotechnology The gap between in vitro and in vivo assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed in vivo. A key challenge in model development, however, is determining how to recreate the multi-cell/system functional complexity of a real network environment that integrates endothelial cells, smooth muscle cells, vascular pericytes, lymphatics, nerves, fluid flow, extracellular matrix, and inflammatory cells. The objective of this mini-review is to overview the recent evolution of popular biomimetic modeling approaches for investigating microvascular dynamics. A specific focus will highlight the engineering design requirements needed to match physiological function and the potential for top-down tissue culture methods that maintain complexity. Overall, examples of physiological validation, basic science discoveries, and therapeutic evaluation studies will emphasize the value of tissue culture models and biomimetic model development approaches that fill the gap between in vitro and in vivo assays and guide how vascular biologists and physiologists might think about the microcirculation. Frontiers Media S.A. 2022-06-20 /pmc/articles/PMC9252339/ /pubmed/35795159 http://dx.doi.org/10.3389/fbioe.2022.912073 Text en Copyright © 2022 Lampejo, Hu, Lucas, Lomel, Nguyen, Dominguez, Ren, Huang and Murfee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Lampejo, Arinola O.
Hu, Nien-Wen
Lucas, Daniela
Lomel, Banks M.
Nguyen, Christian M.
Dominguez, Carmen C.
Ren, Bing
Huang, Yong
Murfee, Walter L.
A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
title A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
title_full A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
title_fullStr A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
title_full_unstemmed A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
title_short A Challenge for Engineering Biomimetic Microvascular Models: How do we Incorporate the Physiology?
title_sort challenge for engineering biomimetic microvascular models: how do we incorporate the physiology?
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252339/
https://www.ncbi.nlm.nih.gov/pubmed/35795159
http://dx.doi.org/10.3389/fbioe.2022.912073
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