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NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis
Pre-exposing (priming) plants to mild, non-lethal elevated temperature improves their tolerance to a later higher-temperature stress (triggering stimulus), which is of great ecological importance. ‘Thermomemory’ is maintaining this tolerance for an extended period of time. NAM/ATAF1/2/CUC2 (NAC) pro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253118/ https://www.ncbi.nlm.nih.gov/pubmed/35787631 http://dx.doi.org/10.1038/s41598-022-14429-x |
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author | Alshareef, Nouf Owdah Otterbach, Sophie L. Allu, Annapurna Devi Woo, Yong H. de Werk, Tobias Kamranfar, Iman Mueller-Roeber, Bernd Tester, Mark Balazadeh, Salma Schmöckel, Sandra M. |
author_facet | Alshareef, Nouf Owdah Otterbach, Sophie L. Allu, Annapurna Devi Woo, Yong H. de Werk, Tobias Kamranfar, Iman Mueller-Roeber, Bernd Tester, Mark Balazadeh, Salma Schmöckel, Sandra M. |
author_sort | Alshareef, Nouf Owdah |
collection | PubMed |
description | Pre-exposing (priming) plants to mild, non-lethal elevated temperature improves their tolerance to a later higher-temperature stress (triggering stimulus), which is of great ecological importance. ‘Thermomemory’ is maintaining this tolerance for an extended period of time. NAM/ATAF1/2/CUC2 (NAC) proteins are plant-specific transcription factors (TFs) that modulate responses to abiotic stresses, including heat stress (HS). Here, we investigated the potential role of NACs for thermomemory. We determined the expression of 104 Arabidopsis NAC genes after priming and triggering heat stimuli, and found ATAF1 expression is strongly induced right after priming and declines below control levels thereafter during thermorecovery. Knockout mutants of ATAF1 show better thermomemory than wild type, revealing a negative regulatory role. Differential expression analyses of RNA-seq data from ATAF1 overexpressor, ataf1 mutant and wild-type plants after heat priming revealed five genes that might be priming-associated direct targets of ATAF1: AT2G31260 (ATG9), AT2G41640 (GT61), AT3G44990 (XTH31), AT4G27720 and AT3G23540. Based on co-expression analyses applied to the aforementioned RNA-seq profiles, we identified ANAC055 to be transcriptionally co-regulated with ATAF1. Like ataf1, anac055 mutants show improved thermomemory, revealing a potential co-control of both NAC TFs over thermomemory. Our data reveals a core importance of two NAC transcription factors, ATAF1 and ANAC055, for thermomemory. |
format | Online Article Text |
id | pubmed-9253118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-92531182022-07-06 NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis Alshareef, Nouf Owdah Otterbach, Sophie L. Allu, Annapurna Devi Woo, Yong H. de Werk, Tobias Kamranfar, Iman Mueller-Roeber, Bernd Tester, Mark Balazadeh, Salma Schmöckel, Sandra M. Sci Rep Article Pre-exposing (priming) plants to mild, non-lethal elevated temperature improves their tolerance to a later higher-temperature stress (triggering stimulus), which is of great ecological importance. ‘Thermomemory’ is maintaining this tolerance for an extended period of time. NAM/ATAF1/2/CUC2 (NAC) proteins are plant-specific transcription factors (TFs) that modulate responses to abiotic stresses, including heat stress (HS). Here, we investigated the potential role of NACs for thermomemory. We determined the expression of 104 Arabidopsis NAC genes after priming and triggering heat stimuli, and found ATAF1 expression is strongly induced right after priming and declines below control levels thereafter during thermorecovery. Knockout mutants of ATAF1 show better thermomemory than wild type, revealing a negative regulatory role. Differential expression analyses of RNA-seq data from ATAF1 overexpressor, ataf1 mutant and wild-type plants after heat priming revealed five genes that might be priming-associated direct targets of ATAF1: AT2G31260 (ATG9), AT2G41640 (GT61), AT3G44990 (XTH31), AT4G27720 and AT3G23540. Based on co-expression analyses applied to the aforementioned RNA-seq profiles, we identified ANAC055 to be transcriptionally co-regulated with ATAF1. Like ataf1, anac055 mutants show improved thermomemory, revealing a potential co-control of both NAC TFs over thermomemory. Our data reveals a core importance of two NAC transcription factors, ATAF1 and ANAC055, for thermomemory. Nature Publishing Group UK 2022-07-04 /pmc/articles/PMC9253118/ /pubmed/35787631 http://dx.doi.org/10.1038/s41598-022-14429-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Alshareef, Nouf Owdah Otterbach, Sophie L. Allu, Annapurna Devi Woo, Yong H. de Werk, Tobias Kamranfar, Iman Mueller-Roeber, Bernd Tester, Mark Balazadeh, Salma Schmöckel, Sandra M. NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis |
title | NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis |
title_full | NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis |
title_fullStr | NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis |
title_full_unstemmed | NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis |
title_short | NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis |
title_sort | nac transcription factors ataf1 and anac055 affect the heat stress response in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253118/ https://www.ncbi.nlm.nih.gov/pubmed/35787631 http://dx.doi.org/10.1038/s41598-022-14429-x |
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