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Engineering plant family TPS into cyanobacterial host for terpenoids production
Terpenoids are synthesized naturally by plants as secondary metabolites, and are diverse and complex in structure with multiple applications in bioenergy, food, cosmetics, and medicine. This makes the production of terpenoids such as isoprene, β-phellandrene, farnesene, amorphadiene, and squalene va...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253243/ https://www.ncbi.nlm.nih.gov/pubmed/35789422 http://dx.doi.org/10.1007/s00299-022-02892-9 |
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author | Rautela, Akhil Kumar, Sanjay |
author_facet | Rautela, Akhil Kumar, Sanjay |
author_sort | Rautela, Akhil |
collection | PubMed |
description | Terpenoids are synthesized naturally by plants as secondary metabolites, and are diverse and complex in structure with multiple applications in bioenergy, food, cosmetics, and medicine. This makes the production of terpenoids such as isoprene, β-phellandrene, farnesene, amorphadiene, and squalene valuable, owing to which their industrial demand cannot be fulfilled exclusively by plant sources. They are synthesized via the Methylerythritol phosphate pathway (MEP) and the Mevalonate pathway (MVA), both existing in plants. The advent of genetic engineering and the latest accomplishments in synthetic biology and metabolic engineering allow microbial synthesis of terpenoids. Cyanobacteria manifest to be the promising hosts for this, utilizing sunlight and CO(2). Cyanobacteria possess MEP pathway to generate precursors for terpenoid synthesis. The terpenoid synthesis can be amplified by overexpressing the MEP pathway and engineering MVA pathway genes. According to the desired terpenoid, terpene synthases unique to the plant kingdom must be incorporated in cyanobacteria. Engineering an organism to be used as a cell factory comes with drawbacks such as hampered cell growth and disturbance in metabolic flux. This review set forth a comparison between MEP and MVA pathways, strategies to overexpress these pathways with their challenges. |
format | Online Article Text |
id | pubmed-9253243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-92532432022-07-05 Engineering plant family TPS into cyanobacterial host for terpenoids production Rautela, Akhil Kumar, Sanjay Plant Cell Rep Review Terpenoids are synthesized naturally by plants as secondary metabolites, and are diverse and complex in structure with multiple applications in bioenergy, food, cosmetics, and medicine. This makes the production of terpenoids such as isoprene, β-phellandrene, farnesene, amorphadiene, and squalene valuable, owing to which their industrial demand cannot be fulfilled exclusively by plant sources. They are synthesized via the Methylerythritol phosphate pathway (MEP) and the Mevalonate pathway (MVA), both existing in plants. The advent of genetic engineering and the latest accomplishments in synthetic biology and metabolic engineering allow microbial synthesis of terpenoids. Cyanobacteria manifest to be the promising hosts for this, utilizing sunlight and CO(2). Cyanobacteria possess MEP pathway to generate precursors for terpenoid synthesis. The terpenoid synthesis can be amplified by overexpressing the MEP pathway and engineering MVA pathway genes. According to the desired terpenoid, terpene synthases unique to the plant kingdom must be incorporated in cyanobacteria. Engineering an organism to be used as a cell factory comes with drawbacks such as hampered cell growth and disturbance in metabolic flux. This review set forth a comparison between MEP and MVA pathways, strategies to overexpress these pathways with their challenges. Springer Berlin Heidelberg 2022-07-05 2022 /pmc/articles/PMC9253243/ /pubmed/35789422 http://dx.doi.org/10.1007/s00299-022-02892-9 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Review Rautela, Akhil Kumar, Sanjay Engineering plant family TPS into cyanobacterial host for terpenoids production |
title | Engineering plant family TPS into cyanobacterial host for terpenoids production |
title_full | Engineering plant family TPS into cyanobacterial host for terpenoids production |
title_fullStr | Engineering plant family TPS into cyanobacterial host for terpenoids production |
title_full_unstemmed | Engineering plant family TPS into cyanobacterial host for terpenoids production |
title_short | Engineering plant family TPS into cyanobacterial host for terpenoids production |
title_sort | engineering plant family tps into cyanobacterial host for terpenoids production |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253243/ https://www.ncbi.nlm.nih.gov/pubmed/35789422 http://dx.doi.org/10.1007/s00299-022-02892-9 |
work_keys_str_mv | AT rautelaakhil engineeringplantfamilytpsintocyanobacterialhostforterpenoidsproduction AT kumarsanjay engineeringplantfamilytpsintocyanobacterialhostforterpenoidsproduction |