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PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway

High concentrations of PM2.5 in enclosed broiler houses cause respiratory disorders in humans and animals. Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that can induce severe respiratory disease in animals under stress or with abnormal immune functions. Alveolar macrophages ar...

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Autores principales: Zhang, Jianlong, Liu, Chong, Zhao, Guangrong, Li, Meng, Ma, Di, Meng, Qingguo, Tang, Wenli, Huang, Qingrong, Shi, Peimin, Li, Youzhi, Jiang, Linlin, Yu, Xin, Zhu, Hongwei, Chen, Guozhong, Zhang, Xingxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253536/
https://www.ncbi.nlm.nih.gov/pubmed/35800443
http://dx.doi.org/10.3389/fphar.2022.924242
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author Zhang, Jianlong
Liu, Chong
Zhao, Guangrong
Li, Meng
Ma, Di
Meng, Qingguo
Tang, Wenli
Huang, Qingrong
Shi, Peimin
Li, Youzhi
Jiang, Linlin
Yu, Xin
Zhu, Hongwei
Chen, Guozhong
Zhang, Xingxiao
author_facet Zhang, Jianlong
Liu, Chong
Zhao, Guangrong
Li, Meng
Ma, Di
Meng, Qingguo
Tang, Wenli
Huang, Qingrong
Shi, Peimin
Li, Youzhi
Jiang, Linlin
Yu, Xin
Zhu, Hongwei
Chen, Guozhong
Zhang, Xingxiao
author_sort Zhang, Jianlong
collection PubMed
description High concentrations of PM2.5 in enclosed broiler houses cause respiratory disorders in humans and animals. Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that can induce severe respiratory disease in animals under stress or with abnormal immune functions. Alveolar macrophages are lung-resident immune cells that play important roles in lung host defence and immune balance. In this study, the mechanism by which PM2.5 synergizes with P. aeruginosa to damage alveolar macrophage function and induce inflammation was investigated. The results will provide a theoretical basis for improving the poultry breeding environment and preventing the recurrence of infection with P. aeruginosa. Alveolar macrophages were stimulated by PM2.5 collected in an enclosed broiler house and P. aeruginosa. Phagocytosis was determined by the neutral red test. The apoptosis rate and cytoskeleton changes were observed by flow cytometry assays and laser scanning confocal microscopy. Protein levels related to autophagy and the mTOR pathway were detected by Western blotting. The results indicated that PM2.5 in combination with P. aeruginosa could decrease phagocytosis, inhibit autophagy, increase apoptosis, and destroy the cytoskeleton in alveolar macrophages. In addition, alveolar macrophages had significantly increased expression of mTOR pathway-related proteins in response to the synergistic stimulation of PM2.5 and P. aeruginosa. The above results confirmed that PM2.5 in poultry houses synergized with P. aeruginosa to impede alveolar macrophage function and caused more severe respiratory system injuries through a process closely related to the activation of the mTOR signalling pathway.
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spelling pubmed-92535362022-07-06 PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway Zhang, Jianlong Liu, Chong Zhao, Guangrong Li, Meng Ma, Di Meng, Qingguo Tang, Wenli Huang, Qingrong Shi, Peimin Li, Youzhi Jiang, Linlin Yu, Xin Zhu, Hongwei Chen, Guozhong Zhang, Xingxiao Front Pharmacol Pharmacology High concentrations of PM2.5 in enclosed broiler houses cause respiratory disorders in humans and animals. Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen that can induce severe respiratory disease in animals under stress or with abnormal immune functions. Alveolar macrophages are lung-resident immune cells that play important roles in lung host defence and immune balance. In this study, the mechanism by which PM2.5 synergizes with P. aeruginosa to damage alveolar macrophage function and induce inflammation was investigated. The results will provide a theoretical basis for improving the poultry breeding environment and preventing the recurrence of infection with P. aeruginosa. Alveolar macrophages were stimulated by PM2.5 collected in an enclosed broiler house and P. aeruginosa. Phagocytosis was determined by the neutral red test. The apoptosis rate and cytoskeleton changes were observed by flow cytometry assays and laser scanning confocal microscopy. Protein levels related to autophagy and the mTOR pathway were detected by Western blotting. The results indicated that PM2.5 in combination with P. aeruginosa could decrease phagocytosis, inhibit autophagy, increase apoptosis, and destroy the cytoskeleton in alveolar macrophages. In addition, alveolar macrophages had significantly increased expression of mTOR pathway-related proteins in response to the synergistic stimulation of PM2.5 and P. aeruginosa. The above results confirmed that PM2.5 in poultry houses synergized with P. aeruginosa to impede alveolar macrophage function and caused more severe respiratory system injuries through a process closely related to the activation of the mTOR signalling pathway. Frontiers Media S.A. 2022-06-21 /pmc/articles/PMC9253536/ /pubmed/35800443 http://dx.doi.org/10.3389/fphar.2022.924242 Text en Copyright © 2022 Zhang, Liu, Zhao, Li, Ma, Meng, Tang, Huang, Shi, Li, Jiang, Yu, Zhu, Chen and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Zhang, Jianlong
Liu, Chong
Zhao, Guangrong
Li, Meng
Ma, Di
Meng, Qingguo
Tang, Wenli
Huang, Qingrong
Shi, Peimin
Li, Youzhi
Jiang, Linlin
Yu, Xin
Zhu, Hongwei
Chen, Guozhong
Zhang, Xingxiao
PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway
title PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway
title_full PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway
title_fullStr PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway
title_full_unstemmed PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway
title_short PM2.5 Synergizes With Pseudomonas aeruginosa to Suppress Alveolar Macrophage Function in Mice Through the mTOR Pathway
title_sort pm2.5 synergizes with pseudomonas aeruginosa to suppress alveolar macrophage function in mice through the mtor pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253536/
https://www.ncbi.nlm.nih.gov/pubmed/35800443
http://dx.doi.org/10.3389/fphar.2022.924242
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