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The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes

Ulcerative colitis (UC) is a serious chronic intestinal inflammatory disease, with an increased incidence in recent years. The intestinal microbiota plays a key role in the pathogenesis of UC. However, there is no unified conclusion on how the intestinal microbiota changes. Most studies focus on the...

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Autores principales: Zhu, Ran, Tang, Junrui, Xing, Chengfeng, Nan, Qiong, Liang, Guili, Luo, Juan, Zhou, Jiao, Miao, Yinglei, Cao, Yu, Dai, Shaoxing, Lan, Danfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253600/
https://www.ncbi.nlm.nih.gov/pubmed/35801108
http://dx.doi.org/10.3389/fmicb.2022.883495
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author Zhu, Ran
Tang, Junrui
Xing, Chengfeng
Nan, Qiong
Liang, Guili
Luo, Juan
Zhou, Jiao
Miao, Yinglei
Cao, Yu
Dai, Shaoxing
Lan, Danfeng
author_facet Zhu, Ran
Tang, Junrui
Xing, Chengfeng
Nan, Qiong
Liang, Guili
Luo, Juan
Zhou, Jiao
Miao, Yinglei
Cao, Yu
Dai, Shaoxing
Lan, Danfeng
author_sort Zhu, Ran
collection PubMed
description Ulcerative colitis (UC) is a serious chronic intestinal inflammatory disease, with an increased incidence in recent years. The intestinal microbiota plays a key role in the pathogenesis of UC. However, there is no unified conclusion on how the intestinal microbiota changes. Most studies focus on the change between UC patients and healthy individuals, rather than the active and remission stage of the same patient. To minimize the influences of genetic differences, environmental and dietary factors, we studied the intestinal microbiota of paired fecal samples from 42 UC patients at the active and remission stages. We identified 175 species of microbes from 11 phyla and found no difference of the alpha and beta diversities between the active and remission stages. Paired t-test analysis revealed differential microbiota at levels of the phyla, class, order, family, genus, and species, including 13 species with differential abundance. For example, CAG-269 sp001916005, Eubacterium F sp003491505, Lachnospira sp000436475, et al. were downregulated in the remission, while the species of Parabacteroides distasonis, Prevotellamassilia sp900540885, CAG-495 sp001917125, et al. were upregulated in the remission. The 13 species can effectively distinguish the active and remission stages. Functional analysis showed that the sporulation and biosynthesis were downregulated, and the hydrogen peroxide catabolic process was upregulated in remission of UC. Our study suggests that the 13 species together may serve as a biomarker panel contributing to identify the active and remission stages of UC, which provides a valuable reference for the treatment of UC patients by FMT or other therapeutic methods.
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spelling pubmed-92536002022-07-06 The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes Zhu, Ran Tang, Junrui Xing, Chengfeng Nan, Qiong Liang, Guili Luo, Juan Zhou, Jiao Miao, Yinglei Cao, Yu Dai, Shaoxing Lan, Danfeng Front Microbiol Microbiology Ulcerative colitis (UC) is a serious chronic intestinal inflammatory disease, with an increased incidence in recent years. The intestinal microbiota plays a key role in the pathogenesis of UC. However, there is no unified conclusion on how the intestinal microbiota changes. Most studies focus on the change between UC patients and healthy individuals, rather than the active and remission stage of the same patient. To minimize the influences of genetic differences, environmental and dietary factors, we studied the intestinal microbiota of paired fecal samples from 42 UC patients at the active and remission stages. We identified 175 species of microbes from 11 phyla and found no difference of the alpha and beta diversities between the active and remission stages. Paired t-test analysis revealed differential microbiota at levels of the phyla, class, order, family, genus, and species, including 13 species with differential abundance. For example, CAG-269 sp001916005, Eubacterium F sp003491505, Lachnospira sp000436475, et al. were downregulated in the remission, while the species of Parabacteroides distasonis, Prevotellamassilia sp900540885, CAG-495 sp001917125, et al. were upregulated in the remission. The 13 species can effectively distinguish the active and remission stages. Functional analysis showed that the sporulation and biosynthesis were downregulated, and the hydrogen peroxide catabolic process was upregulated in remission of UC. Our study suggests that the 13 species together may serve as a biomarker panel contributing to identify the active and remission stages of UC, which provides a valuable reference for the treatment of UC patients by FMT or other therapeutic methods. Frontiers Media S.A. 2022-06-21 /pmc/articles/PMC9253600/ /pubmed/35801108 http://dx.doi.org/10.3389/fmicb.2022.883495 Text en Copyright © 2022 Zhu, Tang, Xing, Nan, Liang, Luo, Zhou, Miao, Cao, Dai and Lan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhu, Ran
Tang, Junrui
Xing, Chengfeng
Nan, Qiong
Liang, Guili
Luo, Juan
Zhou, Jiao
Miao, Yinglei
Cao, Yu
Dai, Shaoxing
Lan, Danfeng
The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes
title The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes
title_full The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes
title_fullStr The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes
title_full_unstemmed The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes
title_short The Distinguishing Bacterial Features From Active and Remission Stages of Ulcerative Colitis Revealed by Paired Fecal Metagenomes
title_sort distinguishing bacterial features from active and remission stages of ulcerative colitis revealed by paired fecal metagenomes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253600/
https://www.ncbi.nlm.nih.gov/pubmed/35801108
http://dx.doi.org/10.3389/fmicb.2022.883495
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