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NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing

Arcobacter species are ubiquitous emerging pathogens with an impact that has been underestimated due to limitations in isolation and detection methods. Our group recently developed the novel NRJ Arcobacter-detection system, with major improvements in specificity and selectivity compared to other cul...

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Autores principales: Nguyen, Paul T., Tuz, Karina, Restaino, Lawrence, Juárez, Oscar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253625/
https://www.ncbi.nlm.nih.gov/pubmed/35801110
http://dx.doi.org/10.3389/fmicb.2022.903079
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author Nguyen, Paul T.
Tuz, Karina
Restaino, Lawrence
Juárez, Oscar
author_facet Nguyen, Paul T.
Tuz, Karina
Restaino, Lawrence
Juárez, Oscar
author_sort Nguyen, Paul T.
collection PubMed
description Arcobacter species are ubiquitous emerging pathogens with an impact that has been underestimated due to limitations in isolation and detection methods. Our group recently developed the novel NRJ Arcobacter-detection system, with major improvements in specificity and selectivity compared to other culture-based methods. In this work, the NRJ detection system was evaluated using retail whole broiler chicken carcass. Nanopore 16S rRNA gene amplicon sequencing demonstrated that Arcobacter species are found in very low abundance in retail chicken and that indigenous microbiota could be a major factor interfering with detection. Comparison of the microbiome obtained from modified Houf broth (HB) method, as the standard detection system, and the novel NRJ method, showed Arcobacter abundances of <15% and >97%, respectively. The NRJ system significantly inhibits the growth of non-target microbiota, and specifically allows the multiplication of Arcobacter species. In this report, we describe the gold-standard of Arcobacter-specific culture-based method to test food matrices, which can be used for other applications, such as clinical and environmental sampling.
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spelling pubmed-92536252022-07-06 NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing Nguyen, Paul T. Tuz, Karina Restaino, Lawrence Juárez, Oscar Front Microbiol Microbiology Arcobacter species are ubiquitous emerging pathogens with an impact that has been underestimated due to limitations in isolation and detection methods. Our group recently developed the novel NRJ Arcobacter-detection system, with major improvements in specificity and selectivity compared to other culture-based methods. In this work, the NRJ detection system was evaluated using retail whole broiler chicken carcass. Nanopore 16S rRNA gene amplicon sequencing demonstrated that Arcobacter species are found in very low abundance in retail chicken and that indigenous microbiota could be a major factor interfering with detection. Comparison of the microbiome obtained from modified Houf broth (HB) method, as the standard detection system, and the novel NRJ method, showed Arcobacter abundances of <15% and >97%, respectively. The NRJ system significantly inhibits the growth of non-target microbiota, and specifically allows the multiplication of Arcobacter species. In this report, we describe the gold-standard of Arcobacter-specific culture-based method to test food matrices, which can be used for other applications, such as clinical and environmental sampling. Frontiers Media S.A. 2022-06-21 /pmc/articles/PMC9253625/ /pubmed/35801110 http://dx.doi.org/10.3389/fmicb.2022.903079 Text en Copyright © 2022 Nguyen, Tuz, Restaino and Juárez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Nguyen, Paul T.
Tuz, Karina
Restaino, Lawrence
Juárez, Oscar
NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing
title NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing
title_full NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing
title_fullStr NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing
title_full_unstemmed NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing
title_short NRJ Media as the Gold-Standard Arcobacter-Specific Detection System: Applications in Poultry Testing
title_sort nrj media as the gold-standard arcobacter-specific detection system: applications in poultry testing
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253625/
https://www.ncbi.nlm.nih.gov/pubmed/35801110
http://dx.doi.org/10.3389/fmicb.2022.903079
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