Identification of lysine-lactylated substrates in gastric cancer cells

Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene express...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Dawei, Yin, Jie, Shan, Liuqun, Yi, Xingling, Zhang, Wei, Ding, Yongbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253728/
https://www.ncbi.nlm.nih.gov/pubmed/35800753
http://dx.doi.org/10.1016/j.isci.2022.104630
_version_ 1784740556069404672
author Yang, Dawei
Yin, Jie
Shan, Liuqun
Yi, Xingling
Zhang, Wei
Ding, Yongbin
author_facet Yang, Dawei
Yin, Jie
Shan, Liuqun
Yi, Xingling
Zhang, Wei
Ding, Yongbin
author_sort Yang, Dawei
collection PubMed
description Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene expression. However, Kla in non-histone mammalian proteins is unclear. Here, a comprehensive analysis of lactylated proteins in gastric cancer AGS cells was conducted. There were 2375 Kla sites found in 1014 proteins. Interestingly, KEGG pathway analysis showed that these proteins were significantly enriched in spliceosome function. In addition, Kla was more abundant in gastric tumors than in adjacent tissues, and high levels of Kla in gastric tumors were associated with poor prognosis. These results suggest that Kla could be a prognostic marker in gastric cancer. This lysine lactylome analysis in gastric cancer cells, the first of its kind, provides a valuable foundation for further studies of Kla.
format Online
Article
Text
id pubmed-9253728
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92537282022-07-06 Identification of lysine-lactylated substrates in gastric cancer cells Yang, Dawei Yin, Jie Shan, Liuqun Yi, Xingling Zhang, Wei Ding, Yongbin iScience Article Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene expression. However, Kla in non-histone mammalian proteins is unclear. Here, a comprehensive analysis of lactylated proteins in gastric cancer AGS cells was conducted. There were 2375 Kla sites found in 1014 proteins. Interestingly, KEGG pathway analysis showed that these proteins were significantly enriched in spliceosome function. In addition, Kla was more abundant in gastric tumors than in adjacent tissues, and high levels of Kla in gastric tumors were associated with poor prognosis. These results suggest that Kla could be a prognostic marker in gastric cancer. This lysine lactylome analysis in gastric cancer cells, the first of its kind, provides a valuable foundation for further studies of Kla. Elsevier 2022-06-17 /pmc/articles/PMC9253728/ /pubmed/35800753 http://dx.doi.org/10.1016/j.isci.2022.104630 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Yang, Dawei
Yin, Jie
Shan, Liuqun
Yi, Xingling
Zhang, Wei
Ding, Yongbin
Identification of lysine-lactylated substrates in gastric cancer cells
title Identification of lysine-lactylated substrates in gastric cancer cells
title_full Identification of lysine-lactylated substrates in gastric cancer cells
title_fullStr Identification of lysine-lactylated substrates in gastric cancer cells
title_full_unstemmed Identification of lysine-lactylated substrates in gastric cancer cells
title_short Identification of lysine-lactylated substrates in gastric cancer cells
title_sort identification of lysine-lactylated substrates in gastric cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253728/
https://www.ncbi.nlm.nih.gov/pubmed/35800753
http://dx.doi.org/10.1016/j.isci.2022.104630
work_keys_str_mv AT yangdawei identificationoflysinelactylatedsubstratesingastriccancercells
AT yinjie identificationoflysinelactylatedsubstratesingastriccancercells
AT shanliuqun identificationoflysinelactylatedsubstratesingastriccancercells
AT yixingling identificationoflysinelactylatedsubstratesingastriccancercells
AT zhangwei identificationoflysinelactylatedsubstratesingastriccancercells
AT dingyongbin identificationoflysinelactylatedsubstratesingastriccancercells