Identification of lysine-lactylated substrates in gastric cancer cells
Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene express...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253728/ https://www.ncbi.nlm.nih.gov/pubmed/35800753 http://dx.doi.org/10.1016/j.isci.2022.104630 |
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author | Yang, Dawei Yin, Jie Shan, Liuqun Yi, Xingling Zhang, Wei Ding, Yongbin |
author_facet | Yang, Dawei Yin, Jie Shan, Liuqun Yi, Xingling Zhang, Wei Ding, Yongbin |
author_sort | Yang, Dawei |
collection | PubMed |
description | Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene expression. However, Kla in non-histone mammalian proteins is unclear. Here, a comprehensive analysis of lactylated proteins in gastric cancer AGS cells was conducted. There were 2375 Kla sites found in 1014 proteins. Interestingly, KEGG pathway analysis showed that these proteins were significantly enriched in spliceosome function. In addition, Kla was more abundant in gastric tumors than in adjacent tissues, and high levels of Kla in gastric tumors were associated with poor prognosis. These results suggest that Kla could be a prognostic marker in gastric cancer. This lysine lactylome analysis in gastric cancer cells, the first of its kind, provides a valuable foundation for further studies of Kla. |
format | Online Article Text |
id | pubmed-9253728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-92537282022-07-06 Identification of lysine-lactylated substrates in gastric cancer cells Yang, Dawei Yin, Jie Shan, Liuqun Yi, Xingling Zhang, Wei Ding, Yongbin iScience Article Cancer cells tend to utilize aerobic glycolysis to generate energy and metabolites; the end product of aerobic glycolysis is lactate, which promotes lysine lactylation (Kla). Kla is a newly discovered histone post-translational modification (PTM) that plays important roles in regulating gene expression. However, Kla in non-histone mammalian proteins is unclear. Here, a comprehensive analysis of lactylated proteins in gastric cancer AGS cells was conducted. There were 2375 Kla sites found in 1014 proteins. Interestingly, KEGG pathway analysis showed that these proteins were significantly enriched in spliceosome function. In addition, Kla was more abundant in gastric tumors than in adjacent tissues, and high levels of Kla in gastric tumors were associated with poor prognosis. These results suggest that Kla could be a prognostic marker in gastric cancer. This lysine lactylome analysis in gastric cancer cells, the first of its kind, provides a valuable foundation for further studies of Kla. Elsevier 2022-06-17 /pmc/articles/PMC9253728/ /pubmed/35800753 http://dx.doi.org/10.1016/j.isci.2022.104630 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Yang, Dawei Yin, Jie Shan, Liuqun Yi, Xingling Zhang, Wei Ding, Yongbin Identification of lysine-lactylated substrates in gastric cancer cells |
title | Identification of lysine-lactylated substrates in gastric cancer cells |
title_full | Identification of lysine-lactylated substrates in gastric cancer cells |
title_fullStr | Identification of lysine-lactylated substrates in gastric cancer cells |
title_full_unstemmed | Identification of lysine-lactylated substrates in gastric cancer cells |
title_short | Identification of lysine-lactylated substrates in gastric cancer cells |
title_sort | identification of lysine-lactylated substrates in gastric cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253728/ https://www.ncbi.nlm.nih.gov/pubmed/35800753 http://dx.doi.org/10.1016/j.isci.2022.104630 |
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