Cargando…

Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens

Gut microbiota plays an important role in health and disease. To determine whether the traditional Chinese formula Zi Huang Huo Xiang San (ZHHXS) modulates gut microbiota under heat stress, a heat stress model was prepared in Roman layer hens by housing them at temperatures of 32–36°C and administer...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Chunxin, Qu, Qian, Bai, Lin, Chen, Jiaxin, Cai, Zhuoke, Sun, Jiaqi, Liu, Cui, Shi, Dayou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253820/
https://www.ncbi.nlm.nih.gov/pubmed/35799842
http://dx.doi.org/10.3389/fvets.2022.905382
_version_ 1784740573474717696
author Ye, Chunxin
Qu, Qian
Bai, Lin
Chen, Jiaxin
Cai, Zhuoke
Sun, Jiaqi
Liu, Cui
Shi, Dayou
author_facet Ye, Chunxin
Qu, Qian
Bai, Lin
Chen, Jiaxin
Cai, Zhuoke
Sun, Jiaqi
Liu, Cui
Shi, Dayou
author_sort Ye, Chunxin
collection PubMed
description Gut microbiota plays an important role in health and disease. To determine whether the traditional Chinese formula Zi Huang Huo Xiang San (ZHHXS) modulates gut microbiota under heat stress, a heat stress model was prepared in Roman layer hens by housing them at temperatures of 32–36°C and administering ZHHXS for 4 weeks. The Roman egg layers were randomly divided into three groups with 10 hens in each: a ZHHXS treatment group (ZHHXS-HS), a heat-stressed group (HS), and a blank control group (BC). The ZHHXS-HS and HS groups were housed in a 34 ± 2°C environment, while the BC group was housed at 25 ± 1°C. The ZHHXS-HS hens were fed a diet supplemented with 1% ZHHXS from 1 to 28 days, while the other groups were not. Gut microbiota in the hens' feces was assessed through 16S rRNA high-throughput sequencing on days 1, 3, 7, 14, and 28. A plot of the PCA scores showed that the gut microbiota composition in the BC group was a similar trend in the ZHHXS-HS group on days 1 and 3. The principal coordinate analysis (PCoA) unweighted distribution showed that the gut microbiota composition had no significant differences between the BC and ZHHXS-HS groups on days 1 and 7. The PCoA weighted distribution showed that the gut microbiota composition had no significant differences between the BC and ZHHXS-HS groups on days 1 and 3. This study showed that the composition of gut microbiota in layer hens with heat stress was modulated by ZHHXS treatment. ZHHXS treatment caused key phylotypes of gut microbiota to match the BC group, particularly Actinobacteria, Bacteroidetes, Bacteroides, and Enterococcus. The effect of ZHHXS in alleviating heat stress could be achieved by altering the composition of gut microbiota and regulating some key phylotypes.
format Online
Article
Text
id pubmed-9253820
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92538202022-07-06 Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens Ye, Chunxin Qu, Qian Bai, Lin Chen, Jiaxin Cai, Zhuoke Sun, Jiaqi Liu, Cui Shi, Dayou Front Vet Sci Veterinary Science Gut microbiota plays an important role in health and disease. To determine whether the traditional Chinese formula Zi Huang Huo Xiang San (ZHHXS) modulates gut microbiota under heat stress, a heat stress model was prepared in Roman layer hens by housing them at temperatures of 32–36°C and administering ZHHXS for 4 weeks. The Roman egg layers were randomly divided into three groups with 10 hens in each: a ZHHXS treatment group (ZHHXS-HS), a heat-stressed group (HS), and a blank control group (BC). The ZHHXS-HS and HS groups were housed in a 34 ± 2°C environment, while the BC group was housed at 25 ± 1°C. The ZHHXS-HS hens were fed a diet supplemented with 1% ZHHXS from 1 to 28 days, while the other groups were not. Gut microbiota in the hens' feces was assessed through 16S rRNA high-throughput sequencing on days 1, 3, 7, 14, and 28. A plot of the PCA scores showed that the gut microbiota composition in the BC group was a similar trend in the ZHHXS-HS group on days 1 and 3. The principal coordinate analysis (PCoA) unweighted distribution showed that the gut microbiota composition had no significant differences between the BC and ZHHXS-HS groups on days 1 and 7. The PCoA weighted distribution showed that the gut microbiota composition had no significant differences between the BC and ZHHXS-HS groups on days 1 and 3. This study showed that the composition of gut microbiota in layer hens with heat stress was modulated by ZHHXS treatment. ZHHXS treatment caused key phylotypes of gut microbiota to match the BC group, particularly Actinobacteria, Bacteroidetes, Bacteroides, and Enterococcus. The effect of ZHHXS in alleviating heat stress could be achieved by altering the composition of gut microbiota and regulating some key phylotypes. Frontiers Media S.A. 2022-06-21 /pmc/articles/PMC9253820/ /pubmed/35799842 http://dx.doi.org/10.3389/fvets.2022.905382 Text en Copyright © 2022 Ye, Qu, Bai, Chen, Cai, Sun, Liu and Shi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Ye, Chunxin
Qu, Qian
Bai, Lin
Chen, Jiaxin
Cai, Zhuoke
Sun, Jiaqi
Liu, Cui
Shi, Dayou
Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens
title Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens
title_full Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens
title_fullStr Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens
title_full_unstemmed Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens
title_short Effect of Traditional Chinese Medicine on the Gut Microbiota in Heat-Stressed Laying Hens
title_sort effect of traditional chinese medicine on the gut microbiota in heat-stressed laying hens
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253820/
https://www.ncbi.nlm.nih.gov/pubmed/35799842
http://dx.doi.org/10.3389/fvets.2022.905382
work_keys_str_mv AT yechunxin effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens
AT quqian effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens
AT bailin effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens
AT chenjiaxin effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens
AT caizhuoke effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens
AT sunjiaqi effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens
AT liucui effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens
AT shidayou effectoftraditionalchinesemedicineonthegutmicrobiotainheatstressedlayinghens