Cargando…

A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies

Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different t...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jun, Kim, Chuna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254108/
https://www.ncbi.nlm.nih.gov/pubmed/35776635
http://dx.doi.org/10.1016/j.xpro.2022.101506
Descripción
Sumario:Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different types of publicly available Drosophila melanogaster datasets obtained using current long-read sequencing technologies. We hope that this protocol will help in guiding researchers who are new to the process of long-read sequencing analysis.