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A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies
Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different t...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254108/ https://www.ncbi.nlm.nih.gov/pubmed/35776635 http://dx.doi.org/10.1016/j.xpro.2022.101506 |
_version_ | 1784740624475357184 |
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author | Kim, Jun Kim, Chuna |
author_facet | Kim, Jun Kim, Chuna |
author_sort | Kim, Jun |
collection | PubMed |
description | Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different types of publicly available Drosophila melanogaster datasets obtained using current long-read sequencing technologies. We hope that this protocol will help in guiding researchers who are new to the process of long-read sequencing analysis. |
format | Online Article Text |
id | pubmed-9254108 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-92541082022-07-06 A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies Kim, Jun Kim, Chuna STAR Protoc Protocol Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different types of publicly available Drosophila melanogaster datasets obtained using current long-read sequencing technologies. We hope that this protocol will help in guiding researchers who are new to the process of long-read sequencing analysis. Elsevier 2022-06-29 /pmc/articles/PMC9254108/ /pubmed/35776635 http://dx.doi.org/10.1016/j.xpro.2022.101506 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Kim, Jun Kim, Chuna A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies |
title | A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies |
title_full | A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies |
title_fullStr | A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies |
title_full_unstemmed | A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies |
title_short | A beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies |
title_sort | beginner’s guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254108/ https://www.ncbi.nlm.nih.gov/pubmed/35776635 http://dx.doi.org/10.1016/j.xpro.2022.101506 |
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